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RAB21, Member RAS Oncogene Family (RAB21) (AA 116-226) antibody

Details for Product No. ABIN395106
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Antigen
Synonyms MGC68816, RAB21, 9630024B22
Epitope
AA 116-226
(14), (7), (3)
Reactivity
Human
(41), (18), (18), (12), (12)
Host
Mouse
(27), (14)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(32), (16), (13), (10), (7), (6), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395106
450.00 $
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Immunogen RAB21 (NP_055814, 116 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1F6
Isotype IgG1 kappa
Specificity RAB21 (NP_055814, 116 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RAB21
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 24348 DA
Gene ID 12406
UniProt NP_055814
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: RAB21 belongs to the RAB family of small GTP-binding proteins that regulate intracellular vesicle targeting (Opdam et al., 2000 [PubMed 10887961]).

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-RAB21, Member RAS Oncogene Family (RAB21) (AA 116-226) antibody Immunofluorescence of monoclonal antibody to RAB21 on HeLa cell (antibody concentration 10 µg/mL). RAB21 monoclonal antibody (M01), clone 1F6 Western Blot analysis of RAB21 expression in HeLa.
anti-RAB21, Member RAS Oncogene Family (RAB21) (AA 116-226) antibody (2) anti-RAB21, Member RAS Oncogene Family (RAB21) (AA 116-226) antibody (Image 2)
Background publications Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Pellinen, Arjonen, Vuoriluoto et al.: "Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins." in: The Journal of cell biology, Vol. 173, Issue 5, pp. 767-80, 2006 (PubMed).

Delprato, Lambright: "Structural basis for Rab GTPase activation by VPS9 domain exchange factors." in: Nature structural & molecular biology, Vol. 14, Issue 5, pp. 406-12, 2007 (PubMed).

Pellinen, Tuomi, Arjonen et al.: "Integrin trafficking regulated by Rab21 is necessary for cytokinesis." in: Developmental cell, Vol. 15, Issue 3, pp. 371-85, 2008 (PubMed).

Burgo, Sotirakis, Simmler et al.: "Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth." in: EMBO reports, Vol. 10, Issue 10, pp. 1117-24, 2009 (PubMed).

Validation Images
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