WD Repeat Domain 4 (WDR4) antibody

Antigen: WD Repeat Domain 4 (WDR4)

Synonyms: TRM82

Clonality: Monoclonal (1F9)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395139

Additional Information

Alternative name: WDR4

Gene ID: 10785

Immunogen: WDR4 (AAH01074, 1 a.a. ~ 267 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1F9

Description: Other names: WD repeat domain 4

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: WDR4 (AAH01074, 1 a.a. ~ 267 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 45490 DA

Comments:

Background: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Two transcript variants encoding the same protein have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

WDR4 monoclonal antibody (M01), clone 1F9 Western Blot analysis of WDR4 expression in HeLa NE ( Cat # L013V3 ). Immunoperoxidase of monoclonal antibody to WDR4 ( Cat # AT4531a ) on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1 ug/ml]

anti-WD Repeat Domain 4 (WDR4) antibody (Image 2)

Publications

Product: Cartlidge, Knebel, Peggie et al.: "The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells." in: The EMBO journal, Vol. 24, Issue 9, pp. 1696-705, 2005 (PubMed).

Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Hu, Warnatz, Vanhecke et al.: "Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins." in: BMC genomics, Vol. 7, pp. 155, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).