Albumin (ALB) antibody

Antigen: Albumin (ALB)

Synonyms: ALB, MGC179277, alb, pro0883, pro0903, pro1341, MGC88928, CSA, DKFZp459F2310, PRO0883, PRO0903, PRO1341, DKFZp779N1935, Alb1, Alb-1, Albza, LOC100136344

Clonality: Monoclonal (1G12-1B3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395163

Additional Information

Alternative name: ALB

Gene ID: 213

Immunogen: ALB (AAH34023, 19 a.a. ~ 610 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1G12-1B3

Description: Other names: DKFZp779N1935, PRO0883, PRO1341 albumin

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ALB (AAH34023, 19 a.a. ~ 610 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 69367 DA

Comments:

Background: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

ALB monoclonal antibody (M01), clone 1G12-1B3 Western Blot analysis of ALB expression in HepG2 ( Cat # L019V1 ). Immunoperoxidase of monoclonal antibody to ALB ( Cat # AT1109a ) on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3ug/ml]

anti-Albumin (ALB) antibody (Image 2)

Publications

Product: Ascenzi, Fasano: "Allostery in a monomeric protein: the case of human serum albumin." in: Biophysical chemistry, Vol. 148, Issue 1-3, pp. 16-22, 2010 (PubMed).

Cheperegin, Efremov, Kozlov: "Precipitation of human serum albumin from yeast culture liquid at pH values below 5." in: Protein expression and purification, Vol. 72, Issue 2, pp. 205-8, 2010 (PubMed).

Martins-de-Souza, Maccarrone, Wobrock et al.: "Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 16, pp. 1176-89, 2010 (PubMed).

Kim, Lee, Jeon et al.: "[Clinical characteristics and ALB gene mutation analysis of Korean patients with bisalbuminemia]." in: The Korean journal of laboratory medicine, Vol. 30, Issue 3, pp. 307-11, 2010 (PubMed).

Schelleman, Brensinger, Chen et al.: "New genetic variant that might improve warfarin dose prediction in African Americans." in: British journal of clinical pharmacology, Vol. 70, Issue 3, pp. 393-9, 2010 (PubMed).