Complement Decay-Accelerating Factor (CD55) antibody

Details for Product No. ABIN395177
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Antigen
Synonyms CR, TC, DAF, CROM
Reactivity
Human
(161), (14), (6), (6), (3), (2), (1)
Host
Mouse
(116), (49), (7), (3), (2), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(23), (16), (15), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(115), (74), (32), (25), (20), (19), (18), (11), (10), (4), (3), (1), (1)
Pubmed 5 references available
Catalog no. ABIN395177
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen DAF (NP_000565, 35 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G3
Isotype IgG1 kappa
Specificity DAF (NP_000565, 35 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DAF
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 41400 DA
Gene ID 12406
UniProt NP_000565
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF), binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Complement Decay-Accelerating Factor (CD55) antibody DAF monoclonal antibody (M01), clone 1G3 Western Blot analysis of DAF expression in HeLa. Detection limit for recombinant GST tagged DAF is approximately 0.03 ng/mL as a capture antibody.
anti-Complement Decay-Accelerating Factor (CD55) antibody (2) anti-Complement Decay-Accelerating Factor (CD55) antibody (Image 2)
Background publications Rajaraman, Brenner, Neta et al.: "Risk of meningioma and common variation in genes related to innate immunity." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 5, pp. 1356-61, 2010 (PubMed).

Han, Lan, Park et al.: "Polymorphisms in innate immunity genes and risk of childhood leukemia." in: Human immunology, Vol. 71, Issue 7, pp. 727-30, 2010 (PubMed).

Romero, Velez Edwards, Kusanovic et al.: "Identification of fetal and maternal single nucleotide polymorphisms in candidate genes that predispose to spontaneous preterm labor with intact membranes." in: American journal of obstetrics and gynecology, Vol. 202, Issue 5, pp. 431.e1-34, 2010 (PubMed).

Gustafsson, Andersson, Hu et al.: "Adenovirus 11p downregulates CD46 early in infection." in: Virology, Vol. 405, Issue 2, pp. 474-82, 2010 (PubMed).

Romero, Friel, Velez Edwards et al.: "A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM)." in: American journal of obstetrics and gynecology, Vol. 203, Issue 4, pp. 361.e1-361.e30, 2010 (PubMed).

Hosts (116), (49), (7), (3), (2), (2), (1)
Reactivities (161), (14), (6), (6), (3), (2), (1)
Applications (115), (74), (32), (25), (20), (19), (18), (11), (10), (4), (3), (1), (1)
Conjugates (23), (16), (15), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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