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SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 1-347)

Details for Product anti-SAE1 Antibody No. ABIN395192, Supplier: Log in to see
Antigen
  • 2400010M20Rik
  • 2610044L12Rik
  • AL033372
  • aos
  • AOS1
  • Aos1p
  • AW743391
  • CDGAP
  • D7Ertd177e
  • HSPC140
  • Sua1
  • SUA1
  • Sua1p
  • UBLE1A
  • Uble1a
Epitope
AA 1-347
39
8
7
6
4
4
2
2
2
2
1
1
1
1
1
Reactivity
Human
130
35
30
10
9
2
2
1
1
1
Host
Mouse
87
27
15
1
Clonality (Clone)
Monoclonal ()
Conjugate
This SAE1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
114
66
36
30
23
12
4
4
4
1
1
1
1
Supplier
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Immunogen SAE1 (AAH00344, 1 a.a. ~ 347 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G4-1G5
Isotype IgG1 lambda
Specificity SAE1 (AAH00344, 1 a.a. ~ 347 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAE1 (SAE1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 38450 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 1-347) (ABIN395192) Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (AA 1-347) (ABIN395192) anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-347) antibody (Image 2)
Background publications Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: , Vol. 3, pp. 89, 2007

Li, Santockyte, Shen, Tekle, Wang, Yang, Chock: "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers." in: Archives of biochemistry and biophysics, Vol. 453, Issue 1, pp. 70-4, 2006

Klenk, Humrich, Quitterer, Lohse: "SUMO-1 controls the protein stability and the biological function of phosducin." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8357-64, 2006

Kimura, Wakamatsu, Suzuki, Ota, Nishikawa, Yamashita, Yamamoto, Sekine, Tsuritani, Wakaguri, Ishii, Sugiyama, Saito, Isono, Irie, Kushida, Yoneyama, Otsuka, Kanda, Yokoi, Kondo, Wagatsuma, Murakawa et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006

Rual, Venkatesan, Hao, Hirozane-Kishikawa, Dricot, Li, Berriz, Gibbons, Dreze, Ayivi-Guedehoussou, Klitgord, Simon, Boxem, Milstein, Rosenberg, Goldberg, Zhang, Wong, Franklin, Li, Albala, Lim et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005