SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-347) antibody

Details for Product No. ABIN395192
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Antigen
Synonyms AOS1, CDGAP, HSPC140, SUA1, UBLE1A, 2400010M20Rik, 2610044L12Rik, AL033372, AW743391, D7Ertd177e, Sua1, Uble1a, Aos1p, Sua1p, aos
Epitope
AA 1-347
(21), (6), (6), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(88), (27), (24), (7), (7), (3)
Host
Mouse
(73), (9), (6), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(78), (59), (24), (20), (11), (10), (3), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN395192
450.00 $
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Immunogen SAE1 (AAH00344, 1 a.a. ~ 347 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G4-1G5
Isotype IgG1 lambda
Specificity SAE1 (AAH00344, 1 a.a. ~ 347 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SAE1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 38450 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-347) antibody Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell (antibody concentration 10 µg/mL). SAE1 monoclonal antibody (M01), clone 1G4-1G5 Western Blot analysis of SAE1 expression in HeLa.
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-347) antibody (2) anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-347) antibody (Image 2)
Background publications Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Klenk, Humrich, Quitterer et al.: "SUMO-1 controls the protein stability and the biological function of phosducin." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8357-64, 2006 (PubMed).

Li, Santockyte, Shen et al.: "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers." in: Archives of biochemistry and biophysics, Vol. 453, Issue 1, pp. 70-4, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

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