SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody

Antigen: SUMO1 Activating Enzyme Subunit 1 (SAE1)

Synonyms: AOS1, SUA1, UBLE1A, FLJ3091, HSPC140

Clonality: Monoclonal (1G4-1G5)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395192

Additional Information

Alternative name: SAE1

Gene ID: 10055

Immunogen: SAE1 (AAH00344, 1 a.a. ~ 347 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 lambda

Clone: 1G4-1G5

Description: Other names: AOS1, HSPC140, SUA1SUMO-1 activating enzyme subunit 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SAE1 (AAH00344, 1 a.a. ~ 347 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 38450 DA

Comments:

Background: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to SAE1 (AT3766a) on HeLa cell . [antibody concentration 10 ug/ml] SAE1 monoclonal antibody (M01), clone 1G4-1G5 Western Blot analysis of SAE1 expression in HeLa ( Cat # L013V1 ).

anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibody (Image 2)

Publications

Product: Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Klenk, Humrich, Quitterer et al.: "SUMO-1 controls the protein stability and the biological function of phosducin." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8357-64, 2006 (PubMed).

Li, Santockyte, Shen et al.: "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers." in: Archives of biochemistry and biophysics, Vol. 453, Issue 1, pp. 70-4, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).