Occludin (OCLN) (AA 423-523) antibody

Details for Product No. ABIN395206
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Antigen
Synonyms tpmt, OCLN, BLCPMG, AI503564, Ocl, ocln, oclnb, wu:fd23h10, wu:fi13c01, zgc:113992, zgc:56359
Epitope
AA 423-523
(28), (7), (6), (4), (4), (2), (2), (1), (1)
Reactivity
Human
(67), (33), (30), (17), (14), (12), (12), (1), (1)
Host
Mouse
(58), (16), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(65), (34), (18), (14), (10), (8), (8), (7), (3), (3), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395206
450.00 $
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Immunogen OCLN (NP_002529, 423 a.a. ~ 523 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1G7
Isotype IgG1 kappa
Specificity OCLN (NP_002529, 423 a.a. ~ 523 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name OCLN
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 59144 DA
Gene ID 12406
NCBI Accession NP_002529
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Occludin (OCLN) (AA 423-523) antibody Immunofluorescence of monoclonal antibody to OCLN on HeLa cell (antibody concentration 10 µg/mL). OCLN monoclonal antibody (M01), clone 1G7 Western Blot analysis of OCLN expression in HL-60.
anti-Occludin (OCLN) (AA 423-523) antibody (2) anti-Occludin (OCLN) (AA 423-523) antibody (Image 2)
Background publications Steed, Rodrigues, Balda et al.: "Identification of MarvelD3 as a tight junction-associated transmembrane protein of the occludin family." in: BMC cell biology, Vol. 10, pp. 95, 2010 (PubMed).

Du, Xu, Yu et al.: "The tight junction protein, occludin, regulates the directional migration of epithelial cells." in: Developmental cell, Vol. 18, Issue 1, pp. 52-63, 2010 (PubMed).

Raleigh, Marchiando, Zhang et al.: "Tight junction-associated MARVEL proteins marveld3, tricellulin, and occludin have distinct but overlapping functions." in: Molecular biology of the cell, Vol. 21, Issue 7, pp. 1200-13, 2010 (PubMed).

Kohaar, Ploss, Korol et al.: "Splicing diversity of the human OCLN gene and its biological significance for hepatitis C virus entry." in: Journal of virology, Vol. 84, Issue 14, pp. 6987-94, 2010 (PubMed).

Raikwar, Vandewalle, Thomas: "Nedd4-2 interacts with occludin to inhibit tight junction formation and enhance paracellular conductance in collecting duct epithelia." in: American journal of physiology. Renal physiology, Vol. 299, Issue 2, pp. F436-44, 2010 (PubMed).

Validation Images
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