Early B-Cell Factor 1 (EBF1) antibody

Antigen: Early B-Cell Factor 1 (EBF1)

Synonyms: EBF, COE1, OLF1, O/E-1, FLJ39389, FLJ41763, Ebf, Olf1, Olf-1, AW990631, 9830166E18Rik, EBF1

Clonality: Monoclonal (1G8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395208

Additional Information

Alternative name: EBF

Gene ID: 1879

Immunogen: EBF (AAH38805, 1 a.a. ~ 592 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1G8

Description: Other names: COE1, EBF1, O/E1, OLF1 early B-cell factor

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: EBF (AAH38805, 1 a.a. ~ 592 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 64464 DA

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of EBF over-expressed 293 cell line, cotransfected with EBF Validated Chimera RNAi ( Cat # H00001879-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EBF monoclonal antibody (M02), clone 1G8 (Cat # AT1844a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of EBF expression in transfected 293T cell line by EBF monoclonal antibody (M02), clone 1G8. Lane 1: EBF transfected lysate(64 KDa). Lane 2: Non-transfected lysate.

anti-Early B-Cell Factor 1 (EBF1) antibody (Image 2)

Publications

Product: Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Roessler, Györy, Imhof et al.: "Distinct promoters mediate the regulation of Ebf1 gene expression by interleukin-7 and Pax5." in: Molecular and cellular biology, Vol. 27, Issue 2, pp. 579-94, 2007 (PubMed).

Sombekke, Arteta, van de Wiel et al.: "Analysis of multiple candidate genes in association with phenotypes of multiple sclerosis." in: Multiple sclerosis (Houndmills, Basingstoke, England), Vol. 16, Issue 6, pp. 652-9, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Okamoto, Ogawa, Nowak et al.: "Genomic profiling of adult acute lymphoblastic leukemia by single nucleotide polymorphism oligonucleotide microarray and comparison to pediatric acute lymphoblastic leukemia." in: Haematologica, Vol. 95, Issue 9, pp. 1481-8, 2010 (PubMed).