Bridging Integrator 1 (BIN1) antibody

Antigen: Bridging Integrator 1 (BIN1)

Synonyms: AMPH2, AMPHL, SH3P9, MGC10367, DKFZp547F068, MGC105358, bin1, MGC53185, amph2, amphl, sh3p9, MGC76187, BIN1, cb57, zgc:86701, MGC128846

Clonality: Monoclonal (1H1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395214

Additional Information

Alternative name: BIN1

Gene ID: 274

UniProt: NP_004296

Immunogen: BIN1 (NP_004296, 355 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 1H1

Description: Other names: AMPH2, AMPHL, DKFZp547F068, MGC10367, SH3P9 bridging integrator 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BIN1 (NP_004296, 355 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 64699 DA

Comments:

Background: This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

BIN1 monoclonal antibody (M01), clone 1H1 Western Blot analysis of BIN1 expression in HeLa NE ( Cat # L013V3 ).

Publications

Product: Claeys, Maisonobe, Böhm et al.: "Phenotype of a patient with recessive centronuclear myopathy and a novel BIN1 mutation." in: Neurology, Vol. 74, Issue 6, pp. 519-21, 2010 (PubMed).

Hong, Smyth, Gao et al.: "BIN1 localizes the L-type calcium channel to cardiac T-tubules." in: PLoS biology, Vol. 8, Issue 2, pp. e1000312, 2010 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).

Seshadri, Fitzpatrick, Ikram et al.: "Genome-wide analysis of genetic loci associated with Alzheimer disease." in: JAMA : the journal of the American Medical Association, Vol. 303, Issue 18, pp. 1832-40, 2010 (PubMed).

Biffi, Anderson, Desikan et al.: "Genetic variation and neuroimaging measures in Alzheimer disease." in: Archives of neurology, Vol. 67, Issue 6, pp. 677-85, 2010 (PubMed).