Lysine (K)-Specific Demethylase 5A (KDM5A) antibody

Antigen: Lysine (K)-Specific Demethylase 5A (KDM5A)

Synonyms: MGC189432, JARID1A, RBP2, RBBP2, Rbbp2, C76986, Jarid1a, AA409370, MGC11659

Clonality: Monoclonal (1H2)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395221

Additional Information

Alternative name: JARID1A

Gene ID: 5927

UniProt: NP_005047

Immunogen: JARID1A (NP_005047, 191 a.a. ~ 291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 1H2

Description: Other names: RBBP2, RBP2 Jumonji, AT rich interactive domain 1A (RBBP2-like)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: JARID1A (NP_005047, 191 a.a. ~ 291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 195816 DA

Comments:

Background: The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged JARID1A is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Hayakawa, Ohtani, Hayakawa et al.: "RBP2 is an MRG15 complex component and down-regulates intragenic histone H3 lysine 4 methylation." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 12, Issue 6, pp. 811-26, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lopez-Bigas, Kisiel, Dewaal et al.: "Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation." in: Molecular cell, Vol. 31, Issue 4, pp. 520-30, 2008 (PubMed).

Wang, Song, Wang et al.: "Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger." in: Nature, Vol. 459, Issue 7248, pp. 847-51, 2009 (PubMed).

Ge, Li, Wang et al.: "Chromatin remodeling: recruitment of histone demethylase RBP2 by Mad1 for transcriptional repression of a Myc target gene, telomerase reverse transcriptase." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 2, pp. 579-86, 2010 (PubMed).