Lysine (K)-Specific Demethylase 5A (KDM5A) antibody

Details for Product No. ABIN395221
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Synonyms RBBP-2, RBBP2, RBP2, AA409370, C76986, Jarid1a, Rbbp2, JARID1A
(13), (2), (2)
(7), (5)
Clonality (Clone)
Monoclonal ()
ELISA, Western Blotting (WB)
(11), (10), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN395221
Quantity 0.1 mg
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Immunogen JARID1A (NP_005047, 191 a.a. ~ 291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1H2
Isotype IgG2a kappa
Specificity JARID1A (NP_005047, 191 a.a. ~ 291 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name JARID1A
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 195816 DA
Gene ID 12406
UniProt NP_005047
Research Area Cell Cycle, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Background: The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Lysine (K)-Specific Demethylase 5A (KDM5A) antibody Detection limit for recombinant GST tagged JARID1A is approximately 0.03 ng/mL as a capture antibody.
Background publications Hayakawa, Ohtani, Hayakawa et al.: "RBP2 is an MRG15 complex component and down-regulates intragenic histone H3 lysine 4 methylation." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 12, Issue 6, pp. 811-26, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lopez-Bigas, Kisiel, Dewaal et al.: "Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation." in: Molecular cell, Vol. 31, Issue 4, pp. 520-30, 2008 (PubMed).

Wang, Song, Wang et al.: "Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger." in: Nature, Vol. 459, Issue 7248, pp. 847-51, 2009 (PubMed).

Ge, Li, Wang et al.: "Chromatin remodeling: recruitment of histone demethylase RBP2 by Mad1 for transcriptional repression of a Myc target gene, telomerase reverse transcriptase." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 2, pp. 579-86, 2010 (PubMed).

Hosts (7), (5)
Reactivities (13), (2), (2)
Applications (11), (10), (2), (1), (1)
Epitopes (3), (2), (2), (1)
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