BRCA1 Associated RING Domain 1 (BARD1) (AA 658-758) antibody

Details for Product No. ABIN395260
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Antigen
Synonyms BARD1, LOC100147913, ENSMUSG00000060893, ENSMUSG00000073653
Epitope
AA 658-758
(12), (8), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(61), (12), (12), (12), (12), (12), (12)
Host
Mouse
(55), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA
(46), (24), (10), (8), (8), (3), (2), (2), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395260
450.00 $
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Immunogen BARD1 (NP_000456, 658 a.a. ~ 758 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2A11
Isotype IgG2a kappa
Specificity BARD1 (NP_000456, 658 a.a. ~ 758 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name BARD1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 86648 DA
Gene ID 12406
NCBI Accession NP_000456
Research Area Cancer, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml
Comment

Background: This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-BRCA1 Associated RING Domain 1 (BARD1) (AA 658-758) antibody Detection limit for recombinant GST tagged BARD1 is approximately 3 ng/mL as a capture antibody.
Background publications Dizin, Irminger-Finger: "Negative feedback loop of BRCA1-BARD1 ubiquitin ligase on estrogen receptor alpha stability and activity antagonized by cancer-associated isoform of BARD1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 5, pp. 693-700, 2010 (PubMed).

De Brakeleer, De Grève, Loris et al.: "Cancer predisposing missense and protein truncating BARD1 mutations in non-BRCA1 or BRCA2 breast cancer families." in: Human mutation, Vol. 31, Issue 3, pp. E1175-85, 2010 (PubMed).

Harte, OBrien, Ryan et al.: "BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription." in: Cancer research, Vol. 70, Issue 6, pp. 2538-47, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).

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