|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BRCA1 Associated RING Domain 1 (BARD1) antibody
|Synonyms||ENSMUSG00000060893, ENSMUSG00000073653, BARD1, LOC100147913|
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||BARD1 (NP_000456, 658 a.a. ~ 758 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: BRCA1 associated RING domain 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||BARD1 (NP_000456, 658 a.a. ~ 758 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||86648 DA|
Background: This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cancer, Cell Structure|
|Restrictions||For Research Use only|
|Detection limit for recombinant GST tagged BARD1 is approximately 3ng/ml as a capture antibody.|
Dizin, Irminger-Finger: "Negative feedback loop of BRCA1-BARD1 ubiquitin ligase on estrogen receptor alpha stability and activity antagonized by cancer-associated isoform of BARD1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 5, pp. 693-700, 2010 (PubMed).
De Brakeleer, De Grève, Loris et al.: "Cancer predisposing missense and protein truncating BARD1 mutations in non-BRCA1 or BRCA2 breast cancer families." in: Human mutation, Vol. 31, Issue 3, pp. E1175-85, 2010 (PubMed).
Harte, OBrien, Ryan et al.: "BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription." in: Cancer research, Vol. 70, Issue 6, pp. 2538-47, 2010 (PubMed).
Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).
Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).
|Hosts||Rabbit (36), Mouse (3)|
|Reactivities||Human (40), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18), Mouse (Murine) (18), Pig (Porcine) (18), Rat (Rattus) (18)|
|Applications||Western Blotting (WB) (20), Immunofluorescence (IF) (15), Immunoprecipitation (IP) (11), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (1), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (2), AA 108-124 (1), AA 743-760 (1)|