BRCA1 Associated RING Domain 1 (BARD1) antibody

Antigen: BRCA1 Associated RING Domain 1 (BARD1)

Synonyms: ENSMUSG00000060893, ENSMUSG00000073653, BARD1, LOC100147913

Clonality: Monoclonal (2A11)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN395260

Additional Information

Alternative name: BARD1

Gene ID: 580

UniProt: NP_000456

Immunogen: BARD1 (NP_000456, 658 a.a. ~ 758 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2A11

Description: Other names: BRCA1 associated RING domain 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: BARD1 (NP_000456, 658 a.a. ~ 758 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 86648 DA

Comments:

Background: This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged BARD1 is approximately 3ng/ml as a capture antibody.

Publications

Product: Dizin, Irminger-Finger: "Negative feedback loop of BRCA1-BARD1 ubiquitin ligase on estrogen receptor alpha stability and activity antagonized by cancer-associated isoform of BARD1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 5, pp. 693-700, 2010 (PubMed).

De Brakeleer, De Grève, Loris et al.: "Cancer predisposing missense and protein truncating BARD1 mutations in non-BRCA1 or BRCA2 breast cancer families." in: Human mutation, Vol. 31, Issue 3, pp. E1175-85, 2010 (PubMed).

Harte, OBrien, Ryan et al.: "BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription." in: Cancer research, Vol. 70, Issue 6, pp. 2538-47, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Liu, Wu, Chen et al.: "A Large-scale genetic association study of esophageal adenocarcinoma risk." in: Carcinogenesis, Vol. 31, Issue 7, pp. 1259-63, 2010 (PubMed).