Dystroglycan 1 (DAG1) antibody

Details for Product No. ABIN395277
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Antigen
Synonyms DG, Dp71, Dp427, D9Wsu13e
Reactivity
Human
(51), (31), (30), (24), (15), (14), (13), (12), (3), (1), (1), (1)
Host
Mouse
(39), (10), (4), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB)
(34), (21), (20), (15), (5), (3), (1), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN395277
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen DAG1 (AAH12740, 31 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2A3
Isotype IgG2a kappa
Specificity DAG1 (AAH12740, 31 a.a. ~ 141 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DAG1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 97581 DA
Gene ID 12406
Research Area Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.

Restrictions For Research Use only
Concentration 1 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Dystroglycan 1 (DAG1) antibody Detection limit for recombinant GST tagged DAG1 is approximately 0.03 ng/mL as a capture antibody.
Background publications Yoshida-Moriguchi, Yu, Stalnaker et al.: "O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding." in: Science (New York, N.Y.), Vol. 327, Issue 5961, pp. 88-92, 2010 (PubMed).

Morgan, Han, Lam et al.: "Association analysis of 3p21 with Crohn's disease in a New Zealand population." in: Human immunology, Vol. 71, Issue 6, pp. 602-9, 2010 (PubMed).

Sgambato, Caredda, Leocata et al.: "Expression of alpha-dystroglycan correlates with tumour grade and predicts survival in oral squamous cell carcinoma." in: Pathology, Vol. 42, Issue 3, pp. 248-54, 2010 (PubMed).

Lara-Chacón, de León, Leocadio et al.: "Characterization of an Importin alpha/beta-recognized nuclear localization signal in beta-dystroglycan." in: Journal of cellular biochemistry, Vol. 110, Issue 3, pp. 706-17, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Hosts (39), (10), (4), (1)
Reactivities (51), (31), (30), (24), (15), (14), (13), (12), (3), (1), (1), (1)
Applications (34), (21), (20), (15), (5), (3), (1), (1), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (13), (4), (3), (3), (3), (1), (1), (1), (1)
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