Alkaline Phosphatase, Placental-Like 2 (ALPPL2) (AA 365-455) antibody

Details for Product No. ABIN395348
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Antigen
Synonyms ALPPL2, ALPG, ALPPL, GCAP, Akp5, C77216, D1Ertd816e, EAP, RGD1565100
Epitope
AA 365-455
(11), (7), (3), (3), (2), (2), (2), (1), (1)
Reactivity
Human
(28), (1), (1), (1)
Host
Mouse
(24), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(27), (10), (9), (5), (5), (3), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395348
450.00 $
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Immunogen ALPPL2 (NP_112603, 365 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2B3
Isotype IgG2a kappa
Specificity ALPPL2 (NP_112603, 365 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ALPPL2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 57377 DA
Gene ID 12406
NCBI Accession NP_112603
Research Area Cell Structure, Cancer
Application Notes Western blot = 1:500-1000
Comment

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Alkaline Phosphatase, Placental-Like 2 (ALPPL2) (AA 365-455) antibody Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody (M07), clone 2B3. Lane 1: ALPPL2 transfected lysate(57.5 KDa). Lane 2: Non-transfected lysate.
anti-Alkaline Phosphatase, Placental-Like 2 (ALPPL2) (AA 365-455) antibody (2) anti-Alkaline Phosphatase, Placental-Like 2 (ALPPL2) (AA 365-455) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Nakano, Shimanuki, Matsushita et al.: "Involvement of intestinal alkaline phosphatase in serum apolipoprotein B-48 level and its association with ABO and secretor blood group types." in: Biochemical and biophysical research communications, Vol. 341, Issue 1, pp. 33-8, 2006 (PubMed).

Pelttari, Winter, Steck et al.: "Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice." in: Arthritis and rheumatism, Vol. 54, Issue 10, pp. 3254-66, 2006 (PubMed).

Validation Images
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