Alkaline Phosphatase, Placental-Like 2 (ALPPL2) antibody

Antigen: Alkaline Phosphatase, Placental-Like 2 (ALPPL2)

Synonyms: ALPG, GCAP, ALPPL, EAP, Akp5, C77216, D1Ertd816e, ALPP, ALPPL2

Clonality: Monoclonal (2B3)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395348

Additional Information

Alternative name: ALPPL2

Gene ID: 251

UniProt: NP_112603

Immunogen: ALPPL2 (NP_112603, 365 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2B3

Description: Other names: ALPG, ALPPL, GCAP alkaline phosphatase, placental-like 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ALPPL2 (NP_112603, 365 a.a. ~ 455 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 57377 DA

Comments:

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi ( Cat # H00000251-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPPL2 monoclonal antibody (M07), clone 2B3 (Cat # AT1125a ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody (M07), clone 2B3. Lane 1: ALPPL2 transfected lysate(57.5 KDa). Lane 2: Non-transfected lysate.

anti-Alkaline Phosphatase, Placental-Like 2 (ALPPL2) antibody (Image 2)

Publications

Product: Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Nakano, Shimanuki, Matsushita et al.: "Involvement of intestinal alkaline phosphatase in serum apolipoprotein B-48 level and its association with ABO and secretor blood group types." in: Biochemical and biophysical research communications, Vol. 341, Issue 1, pp. 33-8, 2006 (PubMed).

Pelttari, Winter, Steck et al.: "Premature induction of hypertrophy during in vitro chondrogenesis of human mesenchymal stem cells correlates with calcification and vascular invasion after ectopic transplantation in SCID mice." in: Arthritis and rheumatism, Vol. 54, Issue 10, pp. 3254-66, 2006 (PubMed).