Zyxin (ZYX) antibody

Details for Product No. ABIN395405
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Antigen
Synonyms ESP-2, HED-2, R75157, 9530098H06Rik, BcDNA:LD06023, CG32018, DLim-1, DmLIM-1, Dmel\\CG32018, Lim102EF, ZYX102, Zyx102, Zyx102EF, lim, zyx102, zyxin, zgc:171680, ZYX, zyx, MGC145270
Reactivity
Human
(71), (36), (33), (26), (25), (24), (12), (1)
Host
Mouse
(57), (11), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(53), (32), (20), (15), (11), (8), (8), (4), (3), (3), (1)
Pubmed 5 references available
Catalog no. ABIN395405
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen ZYX (AAH08743, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C10-4A7
Isotype IgG1 kappa
Specificity ZYX (AAH08743, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ZYX
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 61277 DA
Gene ID 12406
Research Area Cell Signaling, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Zyxin (ZYX) antibody Western blot analysis of ZYX over-expressed 293 cell line, cotransfected with ZYX Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of ZYX expression in transfected 293T cell line by ZYX monoclonal antibody (M01), clone 2C10-4A7. Lane 1: ZYX transfected lysate(61.3 KDa). Lane 2: Non-transfected lysate.
anti-Zyxin (ZYX) antibody (2) anti-Zyxin (ZYX) antibody (Image 2)
Background publications Hirata, Tatsumi, Sokabe: "Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner." in: Journal of cell science, Vol. 121, Issue Pt 17, pp. 2795-804, 2008 (PubMed).

Han, Liu, Profirovic et al.: "Zyxin is involved in thrombin signaling via interaction with PAR-1 receptor." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 12, pp. 4193-206, 2009 (PubMed).

Sun, Huiatt, Paulin et al.: "Synemin interacts with the LIM domain protein zyxin and is essential for cell adhesion and migration." in: Experimental cell research, Vol. 316, Issue 3, pp. 491-505, 2010 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Hosts (57), (11), (3)
Reactivities (71), (36), (33), (26), (25), (24), (12), (1)
Applications (53), (32), (20), (15), (11), (8), (8), (4), (3), (3), (1)
Conjugates (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (17), (12), (6), (5), (3), (3), (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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