RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1) antibody

Antigen: RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1)

Synonyms: GAP, PKWS, RASA, CMAVM, CM-AVM, RASGAP, p120GAP, p120RASGAP, DKFZp434N071, D-RasGAP, RAS-GAP, RasGAP, DmelCG9209, CG9209

Clonality: Monoclonal (2C12)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395417

Additional Information

Alternative name: RASA1

Gene ID: 5921

UniProt: NP_002881

Immunogen: RASA1 (NP_002881, 948 a.a. ~ 1048 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2C12

Description: Other names: CMAVM, DKFZp434N071, GAP, PKWS, RASA, RASGAP, p120GAP RAS p21 protein activator (GTPase activating protein) 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: RASA1 (NP_002881, 948 a.a. ~ 1048 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 116403 DA

Comments:

Background: The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

RASA1 monoclonal antibody (M01), clone 2C12 Western Blot analysis of RASA1 expression in IMR-32 ( Cat # L008V1 ). Immunoperoxidase of monoclonal antibody to RASA1 ( Cat # AT3574a ) on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 6 ug/ml]

anti-RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1) antibody (Image 2)

Publications

Product: Thiex, Mulliken, Revencu et al.: "A novel association between RASA1 mutations and spinal arteriovenous anomalies." in: AJNR. American journal of neuroradiology, Vol. 31, Issue 4, pp. 775-9, 2010 (PubMed).

Bachas, Schuurhuis, Hollink et al.: "High-frequency type I/II mutational shifts between diagnosis and relapse are associated with outcome in pediatric AML: implications for personalized medicine." in: Blood, Vol. 116, Issue 15, pp. 2752-8, 2010 (PubMed).

Anand, Majeti, Acevedo et al.: "MicroRNA-132-mediated loss of p120RasGAP activates the endothelium to facilitate pathological angiogenesis." in: Nature medicine, Vol. 16, Issue 8, pp. 909-14, 2010 (PubMed).

Wiemels, Kang, Chang et al.: "Backtracking RAS mutations in high hyperdiploid childhood acute lymphoblastic leukemia." in: Blood cells, molecules & diseases, Vol. 45, Issue 3, pp. 186-91, 2010 (PubMed).

Hemerly, Bastos, Cerutti: "Identification of several novel non-p.R132 IDH1 variants in thyroid carcinomas." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 163, Issue 5, pp. 747-55, 2010 (PubMed).