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Details for Product No. ABIN395417

RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1) antibody

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Antigen
Synonyms Gap, RasGAP, Rasa, GAPX, CM-AVM, CMAVM, GAP, PKWS, RASA, RASGAP, p120GAP, p120RASGAP, CG9209, D-RasGAP, Dmel\\CG9209, RAS-GAP, RasGap, rasGap
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN395417
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen RASA1 (NP_002881, 948 a.a. ~ 1048 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C12
Isotype IgG1 kappa
Specificity RASA1 (NP_002881, 948 a.a. ~ 1048 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RASA1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 116403 DA
Gene ID 12406
UniProt NP_002881
Research Area Cancer, Cell Cycle, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Thiex, Mulliken, Revencu et al.: "A novel association between RASA1 mutations and spinal arteriovenous anomalies." in: AJNR. American journal of neuroradiology, Vol. 31, Issue 4, pp. 775-9, 2010 (PubMed).

Bachas, Schuurhuis, Hollink et al.: "High-frequency type I/II mutational shifts between diagnosis and relapse are associated with outcome in pediatric AML: implications for personalized medicine." in: Blood, Vol. 116, Issue 15, pp. 2752-8, 2010 (PubMed).

Anand, Majeti, Acevedo et al.: "MicroRNA-132-mediated loss of p120RasGAP activates the endothelium to facilitate pathological angiogenesis." in: Nature medicine, Vol. 16, Issue 8, pp. 909-14, 2010 (PubMed).

Wiemels, Kang, Chang et al.: "Backtracking RAS mutations in high hyperdiploid childhood acute lymphoblastic leukemia." in: Blood cells, molecules & diseases, Vol. 45, Issue 3, pp. 186-91, 2010 (PubMed).

Hemerly, Bastos, Cerutti: "Identification of several novel non-p.R132 IDH1 variants in thyroid carcinomas." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 163, Issue 5, pp. 747-55, 2010 (PubMed).

Alternatives for antigen "RAS P21 Protein Activator (GTPase Activating Protein) 1 (RASA1)", type "Antibodies"
Hosts (50), (15), (1)
Reactivities (64), (36), (35), (27), (24), (24), (24), (1), (1)
Applications (44), (20), (17), (15), (10), (8), (8), (6), (2), (2), (2)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (12), (7), (4), (2), (2), (2), (1), (1), (1), (1)
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