WD Repeat Domain 5 (WDR5) antibody

Antigen: WD Repeat Domain 5 (WDR5)

Synonyms: SWD3, BIG-3, Big, Big-3, AA408785, AA960360, 2410008O07Rik, MGC114572, zgc:56591, zgc:76895, wu:fk47f04, wdr5-a, MGC80538, wdr5-b, MGC81485, big-3, xwdr5, WDR5, MGC137327

Clonality: Monoclonal (2C2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395423

Additional Information

Alternative name: WDR5

Gene ID: 11091

UniProt: NP_060058

Immunogen: WDR5 (NP_060058, 16 a.a. ~ 121 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 2C2

Description: Other names: BIG3WD repeat domain 5

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: WDR5 (NP_060058, 16 a.a. ~ 121 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 36588 DA

Comments:

Background: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Concentration: 1 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to WDR5 (AT4533a) on HeLa cell . [antibody concentration 10 ug/ml] WDR5 monoclonal antibody (M01), clone 2C2. Western Blot analysis of WDR5 expression in A-549 ( Cat # L025V1 ).

anti-WD Repeat Domain 5 (WDR5) antibody (Image 2)

Publications

Product: Wang, Liu, Zhong et al.: "WDR5 is essential for assembly of the VISA-associated signaling complex and virus-triggered IRF3 and NF-kappaB activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 2, pp. 815-20, 2010 (PubMed).

Yates, Menon, Thompson et al.: "Regulation of HOXA2 gene expression by the ATP-dependent chromatin remodeling enzyme CHD8." in: FEBS letters, Vol. 584, Issue 4, pp. 689-93, 2010 (PubMed).

Jin, Zhao, Yi et al.: "c-Myb binds MLL through menin in human leukemia cells and is an important driver of MLL-associated leukemogenesis." in: The Journal of clinical investigation, Vol. 120, Issue 2, pp. 593-606, 2010 (PubMed).

Feng, Yonezawa, Ye et al.: "PHF8 activates transcription of rRNA genes through H3K4me3 binding and H3K9me1/2 demethylation." in: Nature structural & molecular biology, Vol. 17, Issue 4, pp. 445-50, 2010 (PubMed).

Davis, Butcher, Docherty et al.: "A three-stage genome-wide association study of general cognitive ability: hunting the small effects." in: Behavior genetics, Vol. 40, Issue 6, pp. 759-67, 2010 (PubMed).