Vinculin (VCL) (AA 1-1067) antibody

Details for Product No. ABIN395447
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Antigen
Synonyms
VCL, im:6902790, hm:zehn2160, DDBDRAFT_0186762, DDBDRAFT_0232320, DDB_0186762, DDB_0232320, CG3299, CT11081, Dmel\\CG3299, Dmvincp, EG:103B4.1, Vin, Vin2EF, vinc, LOC100228062, CMD1W, CMH15, MV, MVCL, ... show more
VCL, im:6902790, hm:zehn2160, DDBDRAFT_0186762, DDBDRAFT_0232320, DDB_0186762, DDB_0232320, CG3299, CT11081, Dmel\\CG3299, Dmvincp, EG:103B4.1, Vin, Vin2EF, vinc, LOC100228062, CMD1W, CMH15, MV, MVCL, 9430097D22, AA571387, AI462105, AW545629, vcl-A show less
Epitope
AA 1-1067
(23), (8), (7), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(142), (45), (40), (26), (12), (12), (12), (3), (1), (1)
Host
Mouse
(99), (49), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(106), (49), (48), (46), (39), (22), (21), (16), (13), (10), (10), (9), (4), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395447
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Immunogen VCL (AAH39174, 1 a.a. ~ 1067 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C6-1B5
Isotype IgG1 kappa
Specificity VCL (AAH39174, 1 a.a. ~ 1067 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name VCL
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 123799 DA
Gene ID 12406
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Vinculin (VCL) (AA 1-1067) antibody Immunofluorescence of monoclonal antibody to VCL on HeLa cell (antibody concentration 10 µg/mL). VCL monoclonal antibody (M01), clone 2C6-1B5 Western Blot analysis of VCL expression in Hela.
anti-Vinculin (VCL) (AA 1-1067) antibody (2) anti-Vinculin (VCL) (AA 1-1067) antibody (Image 2)
Background publications Peng, Cuff, Lawton et al.: "Vinculin regulates cell-surface E-cadherin expression by binding to beta-catenin." in: Journal of cell science, Vol. 123, Issue Pt 4, pp. 567-77, 2010 (PubMed).

Zimmerman, Cox, Lakdawala et al.: "A novel custom resequencing array for dilated cardiomyopathy." in: Genetics in medicine : official journal of the American College of Medical Genetics, Vol. 12, Issue 5, pp. 268-78, 2010 (PubMed).

Rangarajan, Lee, Yogesha et al.: "A helix replacement mechanism directs metavinculin functions." in: PLoS ONE, Vol. 5, Issue 5, pp. e10679, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Validation Images
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