Coagulation Factor IX (F9) (AA 96-191) antibody

Details for Product No. ABIN395468
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Antigen
Synonyms f9, zgc:109775, fix, ptc, hemb, FIX, HEMB, P19, PTC, THPH8, AW111646, Cf-9, Cf9, FIXA
Epitope
AA 96-191
(7), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(77), (18), (6), (5), (3), (1)
Host
Mouse
(32), (26), (24), (11), (4), (1), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (7), (4), (2), (2), (2)
Application
Western Blotting (WB)
(72), (67), (18), (12), (9), (7), (6), (6), (3), (3), (3), (3), (2), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395468
450.00 $
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Immunogen F9 (NP_000124, 96 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2C9
Isotype IgG1 kappa
Specificity F9 (NP_000124, 96 a.a. ~ 191 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name F9
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 51778 DA
Gene ID 12406
NCBI Accession NP_000124
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Coagulation Factor IX (F9) (AA 96-191) antibody Western blot analysis of F9 over-expressed 293 cell line, cotransfected with F9 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of F9 expression in transfected 293T cell line by F9 monoclonal antibody (M01), clone 2C9. Lane 1: F9 transfected lysate(51.8 KDa). Lane 2: Non-transfected lysate.
anti-Coagulation Factor IX (F9) (AA 96-191) antibody (2) anti-Coagulation Factor IX (F9) (AA 96-191) antibody (Image 2)
Background publications Messer, Velander, Bajaj: "Contribution of magnesium in binding of factor IXa to the phospholipid surface: implications for vitamin K-dependent coagulation proteins." in: Journal of thrombosis and haemostasis : JTH, Vol. 7, Issue 12, pp. 2151-3, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Arellano, Bezemer, Tong et al.: "Gene variants associated with venous thrombosis: confirmation in the MEGA study." in: Journal of thrombosis and haemostasis : JTH, Vol. 8, Issue 5, pp. 1132-4, 2010 (PubMed).

Roberts, Kawut, Krowka et al.: "Genetic risk factors for hepatopulmonary syndrome in patients with advanced liver disease." in: Gastroenterology, Vol. 139, Issue 1, pp. 130-9.e24, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Validation Images
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