gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1) antibody

Antigen: gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1)

Synonyms: GB1, GPRC3A, GABABR1, FLJ92613, GABBR1-3, dJ271M21.1.1, dJ271M21.1.2, GABBR1, MGC140191, GABA(B)R1, DKFZp459L2125

Clonality: Monoclonal (2D7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395531

Additional Information

Alternative name: GABBR1

Gene ID: 2550

Immunogen: GABBR1 (AAH50532, 52 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2D7

Description: Other names: GABAB(1e), GABABR1, GABBR13, GPRC3A, dJ271M21.1.1, dJ271M21.1.2, hGB1a gamma-aminobutyric acid (GABA) B receptor, 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: GABBR1 (AAH50532, 52 a.a. ~ 151 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 108320 DA

Comments:

Background: Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates multiple transcript variants.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to GABBR1 (AT2138a) on HeLa cell . [antibody concentration 10 ug/ml] Western blot analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi ( Cat # H00002550-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody (M01), clone 2D7 (Cat # AT2138a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1) antibody (Image 2)

Publications

Product: Sombekke, Arteta, van de Wiel et al.: "Analysis of multiple candidate genes in association with phenotypes of multiple sclerosis." in: Multiple sclerosis (Houndmills, Basingstoke, England), Vol. 16, Issue 6, pp. 652-9, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Saus, Brunet, Armengol et al.: "Comprehensive copy number variant (CNV) analysis of neuronal pathways genes in psychiatric disorders identifies rare variants within patients." in: Journal of psychiatric research, Vol. 44, Issue 14, pp. 971-8, 2010 (PubMed).

Pinheiro, Bulik, Thornton et al.: "Association study of 182 candidate genes in anorexia nervosa." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 153B, Issue 5, pp. 1070-80, 2010 (PubMed).

Oblak, Gibbs, Blatt: "Decreased GABA(B) receptors in the cingulate cortex and fusiform gyrus in autism." in: Journal of neurochemistry, Vol. 114, Issue 5, pp. 1414-23, 2010 (PubMed).