EH-Domain Containing 2 (EHD2) (AA 365-428) antibody

Details for Product No. ABIN395539
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Antigen
Synonyms MGC64266, EHD1, MGC107790, PAST2, BC027084, C130052H20Rik, MGEPS, fj66d02, wu:fj66d02, zgc:77671
Epitope
AA 365-428
(3), (3), (1), (1)
Reactivity
Human
(13), (5), (5), (2)
Host
Mouse
(5), (4), (4)
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), Western Blotting (WB)
(11), (10), (3), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395539
450.00 $
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Immunogen EHD2 (NP_055416, 365 a.a. ~ 428 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2D8
Isotype IgG1 kappa
Specificity EHD2 (NP_055416, 365 a.a. ~ 428 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EHD2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 61161 DA
Gene ID 12406
NCBI Accession NP_055416
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-EH-Domain Containing 2 (EHD2) (AA 365-428) antibody Immunofluorescence of monoclonal antibody to EHD2 on HeLa cell (antibody concentration 10 µg/mL). Western Blot analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody (M01), clone 2D8. Lane 1: EHD2 transfected lysate(61.2 KDa). Lane 2: Non-transfected lysate.
anti-EH-Domain Containing 2 (EHD2) (AA 365-428) antibody (2) anti-EH-Domain Containing 2 (EHD2) (AA 365-428) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Naslavsky, Caplan: "C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH?" in: Journal of cell science, Vol. 118, Issue Pt 18, pp. 4093-101, 2005 (PubMed).

Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Daumke, Lundmark, Vallis et al.: "Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling." in: Nature, Vol. 449, Issue 7164, pp. 923-927, 2007 (PubMed).

Gudmundsson, Hund, Wright et al.: "EH domain proteins regulate cardiac membrane protein targeting." in: Circulation research, Vol. 107, Issue 1, pp. 84-95, 2010 (PubMed).

Validation Images
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