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EH-Domain Containing 2 (EHD2) (AA 365-428) antibody

Details for Product No. ABIN395539, Supplier: Log in to see
Antigen
  • MGC64266
  • EHD1
  • MGC107790
  • PAST2
  • BC027084
  • C130052H20Rik
  • MGEPS
  • fj66d02
  • wu:fj66d02
  • zgc:77671
Epitope
AA 365-428
16
3
3
3
2
1
Reactivity
Human
35
11
11
3
2
1
1
1
Host
Mouse
17
12
6
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
32
26
7
4
3
1
1
Supplier
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Immunogen EHD2 (NP_055416, 365 a.a. ~ 428 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2D8
Isotype IgG1 kappa
Specificity EHD2 (NP_055416, 365 a.a. ~ 428 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EHD2 (EHD2 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 61161 DA
Gene ID 12406
NCBI Accession NP_055416
Research Area Cell Structure
Pathways
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-EH-Domain Containing 2 (EHD2) (AA 365-428) antibody (ABIN395539) Immunofluorescence of monoclonal antibody to EHD2 on HeLa cell (antibody concentratio...
Immunohistochemistry (IHC) image for anti-EH-Domain Containing 2 (EHD2) (AA 365-428) antibody (ABIN395539) anti-EH-Domain Containing 2 (EHD2) (AA 365-428) antibody (Image 2)
Background publications Gudmundsson, Hund, Wright et al.: "EH domain proteins regulate cardiac membrane protein targeting." in: Circulation research, Vol. 107, Issue 1, pp. 84-95, 2010 (PubMed).

Daumke, Lundmark, Vallis et al.: "Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling." in: Nature, Vol. 449, Issue 7164, pp. 923-927, 2007 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Naslavsky, Caplan: "C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH?" in: Journal of cell science, Vol. 118, Issue Pt 18, pp. 4093-101, 2005 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).