EH-Domain Containing 2 (EHD2) antibody

Details for Product No. ABIN395539
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Synonyms PAST2, BC027084, C130052H20Rik, fj66d02, zgc:77671, wu:fj66d02, MGEPS, MGC64266, EHD1, MGC107790
(11), (5), (5), (2)
(4), (4), (4)
Clonality (Clone)
Monoclonal ()
Immunofluorescence (IF), Western Blotting (WB)
(10), (8), (3), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN395539
Quantity 0.1 mg
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Immunogen EHD2 (NP_055416, 365 a.a. ~ 428 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2D8
Isotype IgG1 kappa
Specificity EHD2 (NP_055416, 365 a.a. ~ 428 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EHD2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 61161 DA
Gene ID 12406
UniProt NP_055416
Research Area Cell Structure
Application Notes Western blot = 1:500-1000

Background: This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-EH-Domain Containing 2 (EHD2) antibody Immunofluorescence of monoclonal antibody to EHD2 on HeLa cell (antibody concentration 10 µg/mL). Western Blot analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody (M01), clone 2D8. Lane 1: EHD2 transfected lysate(61.2 KDa). Lane 2: Non-transfected lysate.
anti-EH-Domain Containing 2 (EHD2) antibody (2) anti-EH-Domain Containing 2 (EHD2) antibody (Image 2)
Background publications Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Naslavsky, Caplan: "C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH?" in: Journal of cell science, Vol. 118, Issue Pt 18, pp. 4093-101, 2005 (PubMed).

Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Daumke, Lundmark, Vallis et al.: "Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling." in: Nature, Vol. 449, Issue 7164, pp. 923-927, 2007 (PubMed).

Gudmundsson, Hund, Wright et al.: "EH domain proteins regulate cardiac membrane protein targeting." in: Circulation research, Vol. 107, Issue 1, pp. 84-95, 2010 (PubMed).

Hosts (4), (4), (4)
Reactivities (11), (5), (5), (2)
Applications (10), (8), (3), (1), (1), (1)
Epitopes (3), (2), (1)
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