MAD2L1 antibody (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast)) (AA 1-206)

Details for Product anti-MAD2L1 Antibody No. ABIN395553, Supplier: Log in to see
Antigen
  • cb408
  • zgc:112133
  • MAD2L1
  • mad2
  • XMad2
  • AA673185
  • MAD2
  • HSMAD2
  • MAD2 mitotic arrest deficient-like 1 (yeast)
  • MAD2 mitotic arrest deficient-like 1
  • MAD2L1
  • mad2l1
  • LOC100218172
  • Mad2l1
Epitope
AA 1-206
32
7
7
6
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
135
36
33
9
6
5
4
4
4
3
3
2
2
1
Host
Mouse
74
46
15
1
Clonality (Clone)
Monoclonal ()
Conjugate
This MAD2L1 antibody is un-conjugated
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
117
46
23
23
23
20
13
7
2
1
1
1
1
1
Options
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Immunogen MAD2L1 (AAH00356, 1 a.a. ~ 206 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E2-1D6
Isotype IgG2b kappa
Specificity MAD2L1 (AAH00356, 1 a.a. ~ 206 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name MAD2L1 (MAD2L1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 23510 DA
Gene ID 12406
Research Area Cell Cycle, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14.

Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-206) antibody (ABIN395553) MAD2L1 monoclonal antibody (M01), clone 2E2-1D6 Western Blot analysis of MAD2L1 expre...
Immunohistochemistry (IHC) image for anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-206) antibody (ABIN395553) anti-MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-206) antibody (Image 2)
Background publications Hewitt, Tighe, Santaguida, White, Jones, Musacchio, Green, Taylor: "Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex." in: The Journal of cell biology, Vol. 190, Issue 1, pp. 25-34, 2010 (PubMed).

Guo, Zhang, Yang, Miao, Shi, Yao, Tan, Sun, Zhao, Yu, Liu, Lin: "Functional evaluation of missense variations in the human MAD1L1 and MAD2L1 genes and their impact on susceptibility to lung cancer." in: Journal of medical genetics, Vol. 47, Issue 9, pp. 616-22, 2010 (PubMed).

Gladhaug, Westgaard, Schjølberg, Burum-Auensen, Pomianowska, Clausen: "Spindle proteins in resected pancreatic head adenocarcinomas: BubR1 is an independent prognostic factor in pancreatobiliary-type tumours." in: Histopathology, Vol. 56, Issue 3, pp. 345-55, 2010 (PubMed).

Wang, Yin, Du, Chen, Liang, Zhang, Du, Wu, Ding, Fan: "Depression of MAD2 inhibits apoptosis and increases proliferation and multidrug resistance in gastric cancer cells by regulating the activation of phosphorylated survivin." in: Tumour biology, Vol. 31, Issue 3, pp. 225-32, 2010 (PubMed).

Kalsi, Kuo, Aliev, Alexander, McMichael, Patterson, Walsh, Zhao, Schuckit, Nurnberger, Edenberg, Kramer, Hesselbrock, Tischfield, Vladimirov, Prescott, Dick, Kendler, Riley: "A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts." in: Human molecular genetics, Vol. 19, Issue 12, pp. 2497-506, 2010 (PubMed).

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