Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-409) antibody

Details for Product No. ABIN395555
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Antigen
Synonyms GAPDS, GAPDHS, GAPD2, GAPDH-2, HSD-35, Gapd-s, Gapds, gapdh-2, cb350, fb71f08, fk58c09, g3pdh, gapdh, gapds, wu:fb71f08, wu:fk58c09, zgc:76908
Epitope
AA 1-409
(6), (4), (2), (1), (1), (1)
Reactivity
Human
(34), (16), (16), (13), (13), (1), (1), (1)
Host
Mouse
(24), (12), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(27), (12), (10), (7), (7), (3), (2), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395555
450.00 $
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Immunogen GAPDS (AAH36373, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E3-2E10
Isotype IgG1 kappa
Specificity GAPDS (AAH36373, 1 a.a. ~ 409 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name GAPDS
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 44501 DA
Gene ID 12406
Research Area Metabolism, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-409) antibody Immunoperoxidase of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis (antibody concentration 1.5 µg/mL). Detection limit for recombinant GST tagged GAPDHS is approximately 1 ng/mL as a capture antibody.
anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-409) antibody (2) anti-Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) (AA 1-409) antibody (Image 2)
Background publications Yatsenko, Roy, Chen et al.: "Non-invasive genetic diagnosis of male infertility using spermatozoal RNA: KLHL10 mutations in oligozoospermic patients impair homodimerization." in: Human molecular genetics, Vol. 15, Issue 23, pp. 3411-9, 2006 (PubMed).

Bertram, McQueen, Mullin et al.: "Systematic meta-analyses of Alzheimer disease genetic association studies: the AlzGene database." in: Nature genetics, Vol. 39, Issue 1, pp. 17-23, 2006 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Shchutskaya, Elkina, Kuravsky et al.: "Investigation of glyceraldehyde-3-phosphate dehydrogenase from human sperms." in: Biochemistry. Biokhimii͡a, Vol. 73, Issue 2, pp. 185-91, 2008 (PubMed).

Corneveaux, Myers, Allen et al.: "Association of CR1, CLU and PICALM with Alzheimer's disease in a cohort of clinically characterized and neuropathologically verified individuals." in: Human molecular genetics, Vol. 19, Issue 16, pp. 3295-301, 2010 (PubMed).

Validation Images
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