Laminin, gamma 1 (Formerly LAMB2) (LAMC1) antibody

Antigen: Laminin, gamma 1 (Formerly LAMB2) (LAMC1)

Clonality: Monoclonal (2E6-B4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395559

Additional Information

Alternative name: LAMC1

Gene ID: 3915

Immunogen: LAMC1 (AAH15586, 1 a.a. ~ 39 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2E6-B4

Description: Other names: LAMB2, MGC87297 laminin, gamma 1 (formerly LAMB2)

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: LAMC1 (AAH15586, 1 a.a. ~ 39 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 4738 DA

Comments:

Background: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Cancer

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged LAMC1 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Ido, Ito, Taniguchi et al.: "Laminin isoforms containing the gamma3 chain are unable to bind to integrins due to the absence of the glutamic acid residue conserved in the C-terminal regions of the gamma1 and gamma2 chains." in: The Journal of biological chemistry, Vol. 283, Issue 42, pp. 28149-57, 2008 (PubMed).

Rooney, Gurpur, Burkin: "Laminin-111 protein therapy prevents muscle disease in the mdx mouse model for Duchenne muscular dystrophy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 19, pp. 7991-6, 2009 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).

Chen, Hill, Schubert et al.: "Is laminin gamma-1 a candidate gene for advanced pelvic organ prolapse?" in: American journal of obstetrics and gynecology, Vol. 202, Issue 5, pp. 505.e1-5, 2010 (PubMed).

Johnatty, Beesley, Chen et al.: "Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"." in: PLoS genetics, Vol. 6, Issue 7, pp. e1001016, 2010 (PubMed).