GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) antibody

Antigen: GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1)

Synonyms: G3BP, HDH-VIII, MGC111040, fj17h05, zgc:56034, wu:fj17h05, g3bp, MGC53271, G3BP1, MGC133963

Clonality: Monoclonal (2F3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395586

Additional Information

Alternative name: G3BP

Gene ID: 10146

Immunogen: G3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2F3

Description: Other names: HDHVIII Ras-GTPase-activating protein SH3-domain-binding protein

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: G3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 52164 DA

Comments:

Background: This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to G3BP (AT2137a) on HeLa cell . [antibody concentration 10 ug/ml] Western blot analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi ( Cat # H00010146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody (M01), clone 2F3 (Cat # AT2137a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

anti-GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1) antibody (Image 2)

Publications

Product: Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Hinton, Myers, Roggero et al.: "The pseudophosphatase MK-STYX interacts with G3BP and decreases stress granule formation." in: The Biochemical journal, Vol. 427, Issue 3, pp. 349-57, 2010 (PubMed).

Shim, Su, Chae et al.: "Epigallocatechin gallate suppresses lung cancer cell growth through Ras-GTPase-activating protein SH3 domain-binding protein 1." in: Cancer prevention research (Philadelphia, Pa.), Vol. 3, Issue 5, pp. 670-9, 2010 (PubMed).

Gao, Ge, Shao et al.: "Tudor-SN interacts with and co-localizes with G3BP in stress granules under stress conditions." in: FEBS letters, Vol. 584, Issue 16, pp. 3525-32, 2010 (PubMed).