rho Guanine Nucleotide Exchange Factor (GEF) 11 (ARHGEF11) antibody

Antigen: rho Guanine Nucleotide Exchange Factor (GEF) 11 (ARHGEF11)

Synonyms: Gtrap48, GTRAP48, KIAA0380, PDZ-RHOGEF, DKFZp667F1223, RhoGEF, MGC90827, mKIAA0380, B930073M02, E130307F09, ARHGEF11

Clonality: Monoclonal (2F4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395591

Additional Information

Alternative name: ARHGEF11

Gene ID: 9826

Immunogen: ARHGEF11 (AAH57394, 651 a.a. ~ 750 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2F4

Description: Other names: GTRAP48, KIAA0380, PDZRHOGEF Rho guanine nucleotide exchange factor (GEF) 11

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ARHGEF11 (AAH57394, 651 a.a. ~ 750 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 167704 DA

Comments:

Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged ARHGEF11 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Zheng, Cierpicki, Momotani et al.: "On the mechanism of autoinhibition of the RhoA-specific nucleotide exchange factor PDZRhoGEF." in: BMC structural biology, Vol. 9, pp. 36, 2009 (PubMed).

Banerjee, Fischer, Wedegaertner: "The amino acid motif L/IIxxFE defines a novel actin-binding sequence in PDZ-RhoGEF." in: Biochemistry, Vol. 48, Issue 33, pp. 8032-43, 2009 (PubMed).

Ying, Giachini, Tostes et al.: "PYK2/PDZ-RhoGEF links Ca2+ signaling to RhoA." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 29, Issue 10, pp. 1657-63, 2009 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Akiyama, Narita, Nakaoka et al.: "Genome-wide association study to identify genetic variants present in Japanese patients harboring intracranial aneurysms." in: Journal of human genetics, Vol. 55, Issue 10, pp. 656-61, 2010 (PubMed).