Ghrelin/obestatin Prepropeptide (GHRL) antibody

Antigen: Ghrelin/obestatin Prepropeptide (GHRL)

Synonyms: MTLRP, obestatin

Clonality: Monoclonal (2F4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395595

Additional Information

Alternative name: GHRL

Gene ID: 51738

Immunogen: GHRL (AAH25791, 1 a.a. ~ 118 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 2F4

Description: Other names: MTLRP, ghrelinghrelin, growth hormone secretagogue receptor ligand

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: GHRL (AAH25791, 1 a.a. ~ 118 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 12911 DA

Comments:

Background: This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake, however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Concentration: 1mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

GHRL monoclonal antibody (M01), clone 2F4. Western Blot analysis of GHRL expression in HeLa. Immunoperoxidase of monoclonal antibody to GHRL ( Cat # AT2199a ) on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

anti-Ghrelin/obestatin Prepropeptide (GHRL) antibody (Image 2)

Publications

Product: Pinheiro, Bulik, Thornton et al.: "Association study of 182 candidate genes in anorexia nervosa." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 153B, Issue 5, pp. 1070-80, 2010 (PubMed).

Zhu, Liang, Zou et al.: "Plasma ghrelin levels and polymorphisms of ghrelin gene in Chinese obese children and adolescents." in: Irish journal of medical science, Vol. 179, Issue 3, pp. 345-9, 2010 (PubMed).

Landgren, Jerlhag, Hallman et al.: "Genetic variation of the ghrelin signaling system in females with severe alcohol dependence." in: Alcoholism, clinical and experimental research, Vol. 34, Issue 9, pp. 1519-24, 2010 (PubMed).

Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).