Acyl-CoA Binding Domain Containing 3 (Acbd3) antibody

Antigen: Acyl-CoA Binding Domain Containing 3 (Acbd3)

Synonyms: PAP7, GCP60, GOCAP1, GOLPH1, Pap7, 60kDa, Gocap1, D1Ertd10e, 8430407O11Rik, zgc:66303, si:dkey-267p15.1, cb366, sb:cb366, wu:fa04b12, wu:fd14b03, wu:fi29h06, ACBD3, acbd3, MGC122176

Clonality: Monoclonal (2G2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395659

Additional Information

Alternative name: ACBD3

Gene ID: 64746

UniProt: NP_073572

Immunogen: ACBD3 (NP_073572, 73 a.a. ~ 172 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2G2

Description: Other names: GCP60, GOCAP1, GOLPH1, PAP7 acyl-Coenzyme A binding domain containing 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ACBD3 (NP_073572, 73 a.a. ~ 172 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 60593 DA

Comments:

Background: The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to ACBD3 (AT1021a) on HeLa cell . [antibody concentration 10 ug/ml] Western Blot analysis of ACBD3 expression in transfected 293T cell line by ACBD3 monoclonal antibody (M01), clone 2G2. Lane 1: ACBD3 transfected lysate(60.6 KDa). Lane 2: Non-transfected lysate.

anti-Acyl-CoA Binding Domain Containing 3 (Acbd3) antibody (Image 2)

Publications

Product: Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Sbodio, Hicks, Simon et al.: "GCP60 preferentially interacts with a caspase-generated golgin-160 fragment." in: The Journal of biological chemistry, Vol. 281, Issue 38, pp. 27924-31, 2006 (PubMed).

Sbodio, Machamer: "Identification of a redox-sensitive cysteine in GCP60 that regulates its interaction with golgin-160." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 29874-81, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Skibola, Bracci, Halperin et al.: "Polymorphisms in the estrogen receptor 1 and vitamin C and matrix metalloproteinase gene families are associated with susceptibility to lymphoma." in: PLoS ONE, Vol. 3, Issue 7, pp. e2816, 2008 (PubMed).