tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) antibody

Antigen: tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB)

Synonyms: HS1, GW128, KCIP-1, 1300003C17Rik, ywhab, MGC53093, MGC78918, 14-3-3beta, hs1, gw128, kcip-1, YWHAB, 1433b, DKFZp459L1720

Clonality: Monoclonal (2G6-3F2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395687

Additional Information

Alternative name: YWHAB

Gene ID: 7529

Immunogen: YWHAB (AAH01359, 1 a.a. ~ 247 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 2G6-3F2

Description: Other names: GW128, HS1, KCIP1 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: YWHAB (AAH01359, 1 a.a. ~ 247 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 28082 DA

Comments:

Background: This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunofluorescence of monoclonal antibody to YWHAB ( Cat # AT4558a ) on HeLa cell. YWHAB monoclonal antibody (M01), clone 2G6-3F2 Western Blot analysis of YWHAB expression in HeLa ( Cat # L013V1 ).

anti-tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, beta Polypeptide (YWHAB) antibody (Image 2)

Publications

Product: Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).

Nord, Segersten, Sandgren et al.: "Focal amplifications are associated with high grade and recurrences in stage Ta bladder carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 6, pp. 1390-402, 2010 (PubMed).

Ohman, Lietzén, Välimäki et al.: "Cytosolic RNA recognition pathway activates 14-3-3 protein mediated signaling and caspase-dependent disruption of cytokeratin network in human keratinocytes." in: Journal of proteome research, Vol. 9, Issue 3, pp. 1549-64, 2010 (PubMed).

Martins-de-Souza, Schmitt, Röder et al.: "Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia." in: Journal of psychiatric research, Vol. 44, Issue 14, pp. 989-91, 2010 (PubMed).

Liu, Tian, Li et al.: "The expression of seven 14-3-3 isoforms in human meningioma." in: Brain research, Vol. 1336, pp. 98-102, 2010 (PubMed).