Acylphosphatase 2, Muscle Type (Acyp2) antibody

Details for Product No. ABIN395739
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Antigen
Synonyms 2310004B09Rik, ACYM, ACYP, AcPDro2, CG18505, Dmel\\CG18505, ACYP2, Acyp2, Mif-ps1, acym, acyp
Reactivity
Human
(7), (3), (3)
Host
Mouse
(4), (3)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
(7), (7), (4), (2)
Pubmed 5 references available
Catalog no. ABIN395739
Quantity 0.1 mg
Price
450.00 $   Plus shipping costs $45.00
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Immunogen ACYP2 (NP_612457, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2H7
Isotype IgG1 kappa
Specificity ACYP2 (NP_612457, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ACYP2
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 11140 DA
Gene ID 12406
UniProt NP_612457
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Acylphosphatase can hydrolyze the phosphoenzyme intermediate of different membrane pumps, particularly the Ca2+/Mg2+-ATPase from sarcoplasmic reticulum of skeletal muscle. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase on the basis of their tissue localization. This gene encodes the muscle-type isoform (MT). An increase of the MT isoform is associated with muscle differentiation.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Acylphosphatase 2, Muscle Type (Acyp2) antibody Western blot analysis of ACYP2 over-expressed 293 cell line, cotransfected with ACYP2 Validated Chimera RNAi used as specificity and loading control. Western Blot analysis of ACYP2 expression in transfected 293T cell line by ACYP2 monoclonal antibody (M06), clone 2H7. Lane 1: ACYP2 transfected lysate(11.1 KDa). Lane 2: Non-transfected lysate.
anti-Acylphosphatase 2, Muscle Type (Acyp2) antibody (2) anti-Acylphosphatase 2, Muscle Type (Acyp2) antibody (Image 2)
Background publications Ng, Hu, Hu et al.: "Replication of a genome-wide case-control study of esophageal squamous cell carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 123, Issue 7, pp. 1610-5, 2008 (PubMed).

Calamai, Tartaglia, Vendruscolo et al.: "Mutational analysis of the aggregation-prone and disaggregation-prone regions of acylphosphatase." in: Journal of molecular biology, Vol. 387, Issue 4, pp. 965-74, 2009 (PubMed).

Oguri, Kato, Yokoi et al.: "Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals." in: American journal of hypertension, Vol. 23, Issue 1, pp. 70-7, 2009 (PubMed).

Yoshida, Kato, Yokoi et al.: "Association of genetic variants with hemorrhagic stroke in Japanese individuals." in: International journal of molecular medicine, Vol. 25, Issue 4, pp. 649-56, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Hosts (4), (3)
Reactivities (7), (3), (3)
Applications (7), (7), (4), (2)
Epitopes (2)
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