|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Quantity||0.1mg (1 mg/ml)|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||WARS (NP_004175, 50 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: GAMMA2, IFI53, IFP53 tryptophanyl-tRNA synthetase|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||WARS (NP_004175, 50 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||53165 DA|
Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunofluorescence|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure|
|Restrictions||For Research Use only|
|Immunofluorescence of monoclonal antibody to WARS (AT4524a) on HeLa cell . [antibody concentration 10 ug/ml] WARS monoclonal antibody (M02), clone 3A12 Western Blot analysis of WARS expression in HeLa ( Cat # L013V1 ). anti-WARS antibody (Image 2)|
Wang, Mao, Zhao et al.: "Increased TTS abrogates IDO-mediated CD4(+) T cells suppression in patients with Graves' disease." in: Endocrine, Vol. 36, Issue 1, pp. 119-25, 2009 (PubMed).
Bhattacharyya, Ghosh, Hansia et al.: "Allostery and conformational free energy changes in human tryptophanyl-tRNA synthetase from essential dynamics and structure networks." in: Proteins, Vol. 78, Issue 3, pp. 506-17, 2010 (PubMed).
Ghanipour, Jirström, Pontén et al.: "The prognostic significance of tryptophanyl-tRNA synthetase in colorectal cancer." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 11, pp. 2949-56, 2009 (PubMed).
Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).
Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).
|Reactivities||Human (2), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Mouse (Murine) (1), Rat (Rattus) (1)|
|Applications||Western Blotting (WB) (3), ELISA (2), Immunohistochemistry (IHC) (2), Flow Cytometry (FACS) (1), Immunofluorescence (IF) (1), Immunoprecipitation (IP) (1)|