|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Barrier To Autointegration Factor 1 (BANF1) antibody
|Synonyms||BAF, BCRP1, D14S1460, MGC111161, Baf, Bcrp1, L2bp1, L2bp1/Baf, MGC105365, BANF1, DKFZp468C097, C78287, baf, banf1, fa01c01, wu:fa01c01|
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: BAF, BCRP1, D14S1460 barrier to autointegration factor 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||BANF1 (AAH05942, 1 a.a. ~ 90 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||10059 DA|
Background: The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml Immunofluorescence|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure|
|Restrictions||For Research Use only|
Li, Craigie: "Virology: HIV goes nuclear." in: Nature, Vol. 441, Issue 7093, pp. 581-2, 2006 (PubMed).
Haraguchi, Koujin, Osakada et al.: "Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells." in: Journal of cell science, Vol. 120, Issue Pt 12, pp. 1967-77, 2007 (PubMed).
Wiebe, Traktman: "Poxviral B1 kinase overcomes barrier to autointegration factor, a host defense against virus replication." in: Cell host & microbe, Vol. 1, Issue 3, pp. 187-97, 2007 (PubMed).
Haraguchi, Kojidani, Koujin et al.: "Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly." in: Journal of cell science, Vol. 121, Issue Pt 15, pp. 2540-54, 2008 (PubMed).
Montes de Oca, Shoemaker, Gucek et al.: "Barrier-to-autointegration factor proteome reveals chromatin-regulatory partners." in: PLoS ONE, Vol. 4, Issue 9, pp. e7050, 2009 (PubMed).
|Hosts||Rabbit (15), Mouse (6)|
|Reactivities||Human (21), Mouse (Murine) (6), Rat (Rattus) (4)|
|Applications||Western Blotting (WB) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunofluorescence (IF) (9), Fluorescence Microscopy (FM) (2), Flow Cytometry (FACS) (1)|
|Epitopes||C-Term (5), N-Term (3), Center (1), Internal Region (1)|