Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-573) antibody

Details for Product No. ABIN395837
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms ME1, zgc:153079, HUMNDME, MES, D9Ertd267e, Mdh-1, Mod-1, Mod1, MOD1, A130037E08Rik, ALF1, HEB, HEBAlt, HTF-4, HTF4, REB, bHLHb20
Epitope
AA 1-573
(7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(45), (27), (27), (13), (13), (12), (1), (1)
Host
Mouse
(41), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(37), (19), (10), (7), (4), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Available
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN395837
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen ME1 (AAH25246, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3B4
Isotype IgG2b kappa
Specificity ME1 (AAH25246, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ME1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 64150 DA
Gene ID 12406
Research Area Metabolism, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-573) antibody Detection limit for recombinant GST tagged ME1 is approximately 3 ng/mL as a capture antibody.
Background publications Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).

Lu, Dollé, Imholz et al.: "Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations." in: Journal of lipid research, Vol. 49, Issue 12, pp. 2582-9, 2008 (PubMed).

Heart, Cline, Collis et al.: "Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion." in: American journal of physiology. Endocrinology and metabolism, Vol. 296, Issue 6, pp. E1354-62, 2009 (PubMed).

Hsieh, Chen, Hung: "Functional roles of the tetramer organization of malic enzyme." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18096-105, 2009 (PubMed).

Hsieh, Su, Ho et al.: "Long-range interaction between the enzyme active site and a distant allosteric site in the human mitochondrial NAD(P)+-dependent malic enzyme." in: Archives of biochemistry and biophysics, Vol. 487, Issue 1, pp. 19-27, 2009 (PubMed).

Validation Images
Did you look for something else?
back to top