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Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-573) antibody

Details for Product No. ABIN395837, Supplier: Log in to see
Antigen
  • ME1
  • zgc:153079
  • HUMNDME
  • MES
  • D9Ertd267e
  • Mdh-1
  • Mod-1
  • Mod1
  • MOD1
  • A130037E08Rik
  • ALF1
  • HEB
  • HEBAlt
  • HTF-4
  • HTF4
  • REB
  • bHLHb20
Epitope
AA 1-573
7
5
4
4
2
2
1
1
1
1
1
1
1
Reactivity
Human
45
19
19
1
1
1
Host
Mouse
38
7
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
Application
ELISA, Western Blotting (WB)
42
22
12
6
5
2
2
1
1
1
1
Supplier
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Immunogen ME1 (AAH25246, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3B4
Isotype IgG2b kappa
Specificity ME1 (AAH25246, 1 a.a. ~ 573 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ME1 (ME1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 64150 DA
Gene ID 12406
Research Area Metabolism, Cell Structure
Pathways
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-573) antibody (ABIN395837) Detection limit for recombinant GST tagged ME1 is approximately 3 ng/mL as a capture ...
Background publications Hsieh, Su, Ho et al.: "Long-range interaction between the enzyme active site and a distant allosteric site in the human mitochondrial NAD(P)+-dependent malic enzyme." in: Archives of biochemistry and biophysics, Vol. 487, Issue 1, pp. 19-27, 2009 (PubMed).

Hsieh, Chen, Hung: "Functional roles of the tetramer organization of malic enzyme." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18096-105, 2009 (PubMed).

Heart, Cline, Collis et al.: "Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion." in: American journal of physiology. Endocrinology and metabolism, Vol. 296, Issue 6, pp. E1354-62, 2009 (PubMed).

Lu, Dollé, Imholz et al.: "Multiple genetic variants along candidate pathways influence plasma high-density lipoprotein cholesterol concentrations." in: Journal of lipid research, Vol. 49, Issue 12, pp. 2582-9, 2008 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).