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NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 1, 7.5kDa (NDUFA1) (AA 24-71) antibody

Details for Product No. ABIN395873, Supplier: Log in to see
Antigen
  • 1810049F12Rik
  • MWFE
  • RGD1560955
  • CI-MWFE
  • ZNF183
Epitope
AA 24-71
5
5
3
3
2
2
1
Reactivity
Human
60
23
21
Host
Mouse
56
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
7
7
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
38
27
11
10
10
5
3
1
Supplier
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Immunogen NDUFA1 (AAH00266, 24 a.a. ~ 71 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3B9-1A1
Isotype IgG1 kappa
Specificity NDUFA1 (AAH00266, 24 a.a. ~ 71 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name NDUFA1 (NDUFA1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 8072 DA
Gene ID 12406
Research Area Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the hydrophobic protein (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Morán, Rivera, Sánchez-Aragó et al.: "Mitochondrial bioenergetics and dynamics interplay in complex I-deficient fibroblasts." in: Biochimica et biophysica acta, Vol. 1802, Issue 5, pp. 443-53, 2010 (PubMed).

Saito, Kawamoto, Kamatani: "Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects." in: Journal of human genetics, Vol. 54, Issue 6, pp. 317-23, 2009 (PubMed).

Potluri, Davila, Ruiz-Pesini et al.: "A novel NDUFA1 mutation leads to a progressive mitochondrial complex I-specific neurodegenerative disease." in: Molecular genetics and metabolism, Vol. 96, Issue 4, pp. 189-95, 2009 (PubMed).

Fernandez-Moreira, Ugalde, Smeets et al.: "X-linked NDUFA1 gene mutations associated with mitochondrial encephalomyopathy." in: Annals of neurology, Vol. 61, Issue 1, pp. 73-83, 2007 (PubMed).

Vogel, Dieteren, van den Heuvel et al.: "Identification of mitochondrial complex I assembly intermediates by tracing tagged NDUFS3 demonstrates the entry point of mitochondrial subunits." in: The Journal of biological chemistry, Vol. 282, Issue 10, pp. 7582-90, 2007 (PubMed).