Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2) antibody

Antigen: Adaptor-Related Protein Complex 1, sigma 2 Subunit (AP1S2)

Synonyms: DC22, MRX59, SIGMA1B, MGC:1902, EST1, AI853648, 1500012A13Rik, zgc:65824, zgc:77925, wu:fb94g06, wu:fd19a08, AP1S2, AP1S1, MGC128457, RGD1561862

Clonality: Monoclonal (3B9-G5)

Host: Mouse

Reactivity: Human

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN395874

Additional Information

Alternative name: AP1S2

Gene ID: 8905

Immunogen: AP1S2 (AAH01117, 1 a.a. ~ 158 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3B9-G5

Description: Other names: DC22, MGC:1902, SIGMA1B adaptor-related protein complex 1, sigma 2 subunit

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: AP1S2 (AAH01117, 1 a.a. ~ 158 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 18615 DA

Comments:

Background: Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of the adaptin protein family. Transcript variants utilizing alternative polyadenylation signals exist for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged AP1S2 is approximately 3ng/ml as a capture antibody.

Publications

Product: Coleman, Van Damme, Day et al.: "Leucine-specific, functional interactions between human immunodeficiency virus type 1 Nef and adaptor protein complexes." in: Journal of virology, Vol. 79, Issue 4, pp. 2066-78, 2005 (PubMed).

Coleman, Hitchin, Noviello et al.: "HIV-1 Nef stabilizes AP-1 on membranes without inducing ARF1-independent de novo attachment." in: Virology, Vol. 345, Issue 1, pp. 148-55, 2006 (PubMed).

Tarpey, Stevens, Teague et al.: "Mutations in the gene encoding the Sigma 2 subunit of the adaptor protein 1 complex, AP1S2, cause X-linked mental retardation." in: American journal of human genetics, Vol. 79, Issue 6, pp. 1119-24, 2006 (PubMed).

Saillour, Zanni, Des Portes et al.: "Mutations in the AP1S2 gene encoding the sigma 2 subunit of the adaptor protein 1 complex are associated with syndromic X-linked mental retardation with hydrocephalus and calcifications in basal ganglia." in: Journal of medical genetics, Vol. 44, Issue 11, pp. 739-44, 2007 (PubMed).

Borck, Mollà-Herman, Boddaert et al.: "Clinical, cellular, and neuropathological consequences of AP1S2 mutations: further delineation of a recognizable X-linked mental retardation syndrome." in: Human mutation, Vol. 29, Issue 7, pp. 966-74, 2008 (PubMed).