Prefoldin Subunit 3 (PFDN3) antibody

Antigen: Prefoldin Subunit 3 (PFDN3)

Clonality: Monoclonal (3D11)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395932

Additional Information

Alternative name: VBP1

Gene ID: 7411

Immunogen: VBP1 (AAH46094, 1 a.a. ~ 198 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3D11

Description: Other names: PFD3, PFDN3, VBP1von Hippel-Lindau binding protein 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: VBP1 (AAH46094, 1 a.a. ~ 198 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 22658 DA

Comments:

Background: The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Concentration: 1mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

VBP1 monoclonal antibody (M01), clone 3D11 Western Blot analysis of VBP1 expression in HL-60 ( Cat # L014V1 ). Immunoperoxidase of monoclonal antibody to VBP1 ( Cat # AT4504a ) on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 5ug/ml]

anti-Prefoldin Subunit 3 (PFDN3) antibody (Image 2)

Publications

Product: Kralovics, Skoda: "Molecular pathogenesis of Philadelphia chromosome negative myeloproliferative disorders." in: Blood reviews, Vol. 19, Issue 1, pp. 1-13, 2004 (PubMed).

Ross, Grafham, Coffey et al.: "The DNA sequence of the human X chromosome." in: Nature, Vol. 434, Issue 7031, pp. 325-37, 2005 (PubMed).

Kimura, Wakamatsu, Suzuki et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Mousnier, Kubat, Massias-Simon et al.: "von Hippel Lindau binding protein 1-mediated degradation of integrase affects HIV-1 gene expression at a postintegration step." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 34, pp. 13615-20, 2007 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).