Fatty Acid Desaturase 3 (FADS3) antibody

Antigen: Fatty Acid Desaturase 3 (FADS3)

Synonyms: CYB5RP, LLCDL3, AI464531, FADS3, MGC155279, ENDOPLASMIC RETICULUM 18:2 DESATURASE, F23F1.10, F23F1_10, FAD3, FATTY ACID DESATURASE 3, OMEGA-3 FATTY ACID DESATURASE

Clonality: Monoclonal (3D2)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395939

Additional Information

Alternative name: FADS3

Gene ID: 3995

UniProt: NP_068373

Immunogen: FADS3 (NP_068373, 16 a.a. ~ 114 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3D2

Description: Other names: BC269730_1, CYB5RP, LLCDL3 fatty acid desaturase 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: FADS3 (NP_068373, 16 a.a. ~ 114 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 51145 DA

Comments:

Background: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1, this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged FADS3 is approximately 0.1ng/ml as a capture antibody.

Publications

Product: Pédrono, Blanchard, Kloareg et al.: "The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues." in: Journal of lipid research, Vol. 51, Issue 3, pp. 472-9, 2010 (PubMed).

Hicks, Pramstaller, Johansson et al.: "Genetic determinants of circulating sphingolipid concentrations in European populations." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000672, 2009 (PubMed).

Talmud, Drenos, Shah et al.: "Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip." in: American journal of human genetics, Vol. 85, Issue 5, pp. 628-42, 2009 (PubMed).

Mathias, Vergara, Gao et al.: "FADS genetic variants and omega-6 polyunsaturated fatty acid metabolism in a homogeneous island population." in: Journal of lipid research, Vol. 51, Issue 9, pp. 2766-74, 2010 (PubMed).

Bailey, Xie, Do et al.: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).