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RHOH (AA 1-192) antibody

Details for Product No. ABIN395948, Supplier: Log in to see
Antigen
  • ARHH
  • TTF
  • 5830400A04Rik
  • AU019774
  • Arhh
Epitope
AA 1-192
25
17
5
4
1
1
1
Reactivity
Human
47
13
13
2
Host
Mouse
41
6
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
ELISA
38
33
14
9
5
1
1
Supplier
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Immunogen RHOH (AAH14261, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D3
Isotype IgG2a kappa
Specificity RHOH (AAH14261, 1 a.a. ~ 192 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 21331 DA
Gene ID 12406
Research Area Cancer, Cell Structure
Pathways
Application Notes ELISA ~~ 1ug/ml~3ng/ml
Comment

Background: The protein encoded by this gene is a member of the Ras superfamily of small GTPases. Expression of a chimeric transcript of LAZ3 and this gene has been reported as a result of the translocation t(3,4) in non-Hodgkin's lymphomas. This gene encodes a small G-like protein, and unlike most other small G proteins which are expressed ubiquitously, this gene is transcribed only in hemopoietic cells.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
ELISA image for anti-RHOH (AA 1-192) antibody (ABIN395948) Detection limit for recombinant GST tagged RHOH is approximately 1 ng/mL as a capture...
Background publications Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Schmidt-Mende, Geering, Yousefi et al.: "Lysosomal degradation of RhoH protein upon antigen receptor activation in T but not B cells." in: European journal of immunology, Vol. 40, Issue 2, pp. 525-9, 2010 (PubMed).

Sanchez-Aguilera, Rattmann, Drew et al.: "Involvement of RhoH GTPase in the development of B-cell chronic lymphocytic leukemia." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 24, Issue 1, pp. 97-104, 2010 (PubMed).

Daryadel, Yousefi, Troi et al.: "RhoH/TTF negatively regulates leukotriene production in neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 10, pp. 6527-32, 2009 (PubMed).

Iwasaki, Katsumi, Kiyoi et al.: "Prognostic implication and biological roles of RhoH in acute myeloid leukaemia." in: European journal of haematology, Vol. 81, Issue 6, pp. 454-60, 2008 (PubMed).