Src Kinase Associated phosphoprotein 1 (SKAP1) (AA 1-359) antibody

Details for Product No. ABIN395949
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Antigen
Synonyms 1700091G21Rik, Scap1, Skap-55, SCAP1, SKAP55, Skap55
Epitope
AA 1-359
(7), (6), (6), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(47), (16), (4)
Host
Mouse
(36), (11), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(35), (20), (10), (6), (6), (2), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395949
450.00 $
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Immunogen SCAP1 (AAH47870, 1 a.a. ~ 359 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D3
Isotype IgG2a kappa
Specificity SCAP1 (AAH47870, 1 a.a. ~ 359 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SCAP1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 41462 DA
Gene ID 12406
Research Area Cell Signaling, Cell Structure
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml
Comment

Background: This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Src Kinase Associated phosphoprotein 1 (SKAP1) (AA 1-359) antibody Immunoperoxidase of monoclonal antibody to SCAP1 on formalin-fixed paraffin-embedded human tonsil (antibody concentration 3 µg/mL).
Background publications Kosco, Cerignoli, Williams et al.: "SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1." in: Molecular immunology, Vol. 45, Issue 2, pp. 510-22, 2007 (PubMed).

Fang, Seki, Fang: "SKAP associates with kinetochores and promotes the metaphase-to-anaphase transition." in: Cell cycle (Georgetown, Tex.), Vol. 8, Issue 17, pp. 2819-27, 2009 (PubMed).

Lehmann, Meyer, Schuemann et al.: "A novel S3S-TAP-tag for the isolation of T-cell interaction partners of adhesion and degranulation promoting adaptor protein." in: Proteomics, Vol. 9, Issue 23, pp. 5288-95, 2009 (PubMed).

Raab, Wang, Lu et al.: "T cell receptor "inside-out" pathway via signaling module SKAP1-RapL regulates T cell motility and interactions in lymph nodes." in: Immunity, Vol. 32, Issue 4, pp. 541-56, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).

Validation Images
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