Src Kinase Associated phosphoprotein 1 (SKAP1) antibody

Antigen: Src Kinase Associated phosphoprotein 1 (SKAP1)

Clonality: Monoclonal (3D3)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395949

Additional Information

Alternative name: SCAP1

Gene ID: 8631

Immunogen: SCAP1 (AAH47870, 1 a.a. ~ 359 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3D3

Description: Other names: SKAP55 src family associated phosphoprotein 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: SCAP1 (AAH47870, 1 a.a. ~ 359 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 41462 DA

Comments:

Background: This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to SCAP1 ( Cat # AT3781a ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Publications

Product: Kosco, Cerignoli, Williams et al.: "SKAP55 modulates T cell antigen receptor-induced activation of the Ras-Erk-AP1 pathway by binding RasGRP1." in: Molecular immunology, Vol. 45, Issue 2, pp. 510-22, 2007 (PubMed).

Fang, Seki, Fang: "SKAP associates with kinetochores and promotes the metaphase-to-anaphase transition." in: Cell cycle (Georgetown, Tex.), Vol. 8, Issue 17, pp. 2819-27, 2009 (PubMed).

Lehmann, Meyer, Schuemann et al.: "A novel S3S-TAP-tag for the isolation of T-cell interaction partners of adhesion and degranulation promoting adaptor protein." in: Proteomics, Vol. 9, Issue 23, pp. 5288-95, 2009 (PubMed).

Raab, Wang, Lu et al.: "T cell receptor "inside-out" pathway via signaling module SKAP1-RapL regulates T cell motility and interactions in lymph nodes." in: Immunity, Vol. 32, Issue 4, pp. 541-56, 2010 (PubMed).

Rose, Behm, Drgon et al.: "Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score." in: Molecular medicine (Cambridge, Mass.), Vol. 16, Issue 7-8, pp. 247-53, 2010 (PubMed).