Killer Cell Lectin-Like Receptor Subfamily C, Member 3 (KLRC3) antibody

Antigen: Killer Cell Lectin-Like Receptor Subfamily C, Member 3 (KLRC3)

Synonyms: NKG2E, NKG2-E, Nkg2e, KLRC3, KLRC2, NKG2-C

Clonality: Monoclonal (3D5)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395957

Additional Information

Alternative name: KLRC3

Gene ID: 3823

UniProt: NP_002252

Immunogen: KLRC3 (NP_002252, 132 a.a. ~ 241 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2a kappa

Clone: 3D5

Description: Other names: NKG2E, NKG2E killer cell lectin-like receptor subfamily C, member 3

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: KLRC3 (NP_002252, 132 a.a. ~ 241 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 27012 DA

Comments:

Background: Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. KLRC3 is a member of the NKG2 group which are expressed primarily in natural killer (NK) cells and encodes a family of transmembrane proteins characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed on NK cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cancer

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged KLRC3 is approximately 0.3ng/ml as a capture antibody.

Publications

Product: Lazetic, Chang, Houchins et al.: "Human natural killer cell receptors involved in MHC class I recognition are disulfide-linked heterodimers of CD94 and NKG2 subunits." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 11, pp. 4741-5, 1996 (PubMed).

Plougastel, Trowsdale: "Sequence analysis of a 62-kb region overlapping the human KLRC cluster of genes." in: Genomics, Vol. 49, Issue 2, pp. 193-9, 1998 (PubMed).

Glienke, Sobanov, Brostjan et al.: "The genomic organization of NKG2C, E, F, and D receptor genes in the human natural killer gene complex." in: Immunogenetics, Vol. 48, Issue 3, pp. 163-73, 1998 (PubMed).

Bellón, Heredia, Llano et al.: "Triggering of effector functions on a CD8+ T cell clone upon the aggregation of an activatory CD94/kp39 heterodimer." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 7, pp. 3996-4002, 1999 (PubMed).

Shum, Flodin, Muir et al.: "Conservation and variation in human and common chimpanzee CD94 and NKG2 genes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 1, pp. 240-52, 2001 (PubMed).