Integrator Complex Subunit 6 (INTS6) antibody

Antigen: Integrator Complex Subunit 6 (INTS6)

Synonyms: HDB, INT6, DBI-1, DDX26, DICE1, DDX26A, Notchl2, DKFZp434B105, Ddx26, Notch2l, AI480962, 2900075H24Rik, LRRGT00024, ints6, MGC63527, zgc:63527, INTS6, ints6-b

Clonality: Monoclonal (3D9)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395973

Additional Information

Alternative name: DDX26

Gene ID: 26512

UniProt: NP_036273

Immunogen: DDX26 (NP_036273, 779 a.a. ~ 888 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG1 kappa

Clone: 3D9

Description: Other names: DBI1, DDX26A, DICE1, DKFZP434B105, HDB, Notchl2 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 26

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: DDX26 (NP_036273, 779 a.a. ~ 888 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 100390 DA

Comments:

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and located in the critical region of loss of heterozygosity (LOH). Three transcript variants encoding two different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000 Immunohistochemistry ~~ 1.5ug/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Immunoperoxidase of monoclonal antibody to DDX26 ( Cat # AT1734a ) on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

Publications

Product: Röpke, Buhtz, Böhm et al.: "Promoter CpG hypermethylation and downregulation of DICE1 expression in prostate cancer." in: Oncogene, Vol. 24, Issue 44, pp. 6667-75, 2005 (PubMed).

Baillat, Hakimi, Näär et al.: "Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II." in: Cell, Vol. 123, Issue 2, pp. 265-76, 2005 (PubMed).

Hernández, Papadopoulos, Almeida: "Absence of mutations in DICE1/DDX26 gene in human cancer cell lines with frequent 13q14 deletions." in: Cancer genetics and cytogenetics, Vol. 163, Issue 1, pp. 91-2, 2005 (PubMed).

Buchsbaum, Morris, Bochard et al.: "Human INT6 interacts with MCM7 and regulates its stability during S phase of the cell cycle." in: Oncogene, Vol. 26, Issue 35, pp. 5132-44, 2007 (PubMed).

Barbe, Lundberg, Oksvold et al.: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008 (PubMed).