Actinin, alpha 1 (ACTN1) (AA 543-640) antibody

Details for Product No. ABIN395990
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Antigen
Synonyms ACTN1, actinin, BDPLT15, 3110023F10Rik, Actn1a
Epitope
AA 543-640
(14), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(40), (14), (14), (5), (3), (1), (1), (1), (1), (1), (1)
Host
Mouse
(30), (17)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(45), (19), (13), (9), (7), (7), (7), (5), (5), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN395990
450.00 $
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Immunogen ACTN1 (NP_001093, 543 a.a. ~ 640 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3F1
Isotype IgG3 kappa
Specificity ACTN1 (NP_001093, 543 a.a. ~ 640 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name ACTN1
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 103058 DA
Gene ID 12406
NCBI Accession NP_001093
Research Area Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-Actinin, alpha 1 (ACTN1) (AA 543-640) antibody Western Blot analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1. Lane 1: ACTN1 transfected lysate(103.1 KDa). Lane 2: Non-transfected lysate.
Background publications Caporaso, Gu, Chatterjee et al.: "Genome-wide and candidate gene association study of cigarette smoking behaviors." in: PLoS ONE, Vol. 4, Issue 2, pp. e4653, 2009 (PubMed).

Jevnikar, Obermajer, Pecar-Fonović et al.: "Cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding." in: European journal of immunology, Vol. 39, Issue 11, pp. 3217-27, 2010 (PubMed).

Sen, Dong, Kumar: "Isoform-specific contributions of alpha-actinin to glioma cell mechanobiology." in: PLoS ONE, Vol. 4, Issue 12, pp. e8427, 2009 (PubMed).

Quick, Skalli: "Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells." in: Experimental cell research, Vol. 316, Issue 7, pp. 1137-47, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).

Validation Images
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