Actinin, alpha 1 (ACTN1) antibody

Antigen: Actinin, alpha 1 (ACTN1)

Synonyms: FLJ40884, FLJ54432, Actn1a, 3110023F10Rik, ACTN1, MGC128689, MGC147142

Clonality: Monoclonal (3F1)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN395990

Additional Information

Alternative name: ACTN1

Gene ID: 87

UniProt: NP_001093

Immunogen: ACTN1 (NP_001093, 543 a.a. ~ 640 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG3 kappa

Clone: 3F1

Description: Other names: actinin, alpha 1

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: ACTN1 (NP_001093, 543 a.a. ~ 640 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 103058 DA

Comments:

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western Blot analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1. Lane 1: ACTN1 transfected lysate(103.1 KDa). Lane 2: Non-transfected lysate.

Publications

Product: Caporaso, Gu, Chatterjee et al.: "Genome-wide and candidate gene association study of cigarette smoking behaviors." in: PLoS ONE, Vol. 4, Issue 2, pp. e4653, 2009 (PubMed).

Jevnikar, Obermajer, Pecar-Fonović et al.: "Cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding." in: European journal of immunology, Vol. 39, Issue 11, pp. 3217-27, 2010 (PubMed).

Sen, Dong, Kumar: "Isoform-specific contributions of alpha-actinin to glioma cell mechanobiology." in: PLoS ONE, Vol. 4, Issue 12, pp. e8427, 2009 (PubMed).

Quick, Skalli: "Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells." in: Experimental cell research, Vol. 316, Issue 7, pp. 1137-47, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).