|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Actinin, alpha 1 (ACTN1) antibody
|Synonyms||FLJ40884, FLJ54432, Actn1a, 3110023F10Rik, ACTN1, MGC128689, MGC147142|
Alternatives ELISA, Western Blotting (WB)
|5 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||ACTN1 (NP_001093, 543 a.a. ~ 640 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K|
|Description||Other names: actinin, alpha 1|
|Characteristics||Purified Mouse Monoclonal Antibody (Mab)|
|Specificity||ACTN1 (NP_001093, 543 a.a. ~ 640 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Molecular Weight||103058 DA|
Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
|Application Notes||ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000|
|Buffer||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure|
|Restrictions||For Research Use only|
|Western Blot analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1. Lane 1: ACTN1 transfected lysate(103.1 KDa). Lane 2: Non-transfected lysate.|
Caporaso, Gu, Chatterjee et al.: "Genome-wide and candidate gene association study of cigarette smoking behaviors." in: PLoS ONE, Vol. 4, Issue 2, pp. e4653, 2009 (PubMed).
Jevnikar, Obermajer, Pecar-Fonović et al.: "Cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding." in: European journal of immunology, Vol. 39, Issue 11, pp. 3217-27, 2010 (PubMed).
Sen, Dong, Kumar: "Isoform-specific contributions of alpha-actinin to glioma cell mechanobiology." in: PLoS ONE, Vol. 4, Issue 12, pp. e8427, 2009 (PubMed).
Quick, Skalli: "Alpha-actinin 1 and alpha-actinin 4: contrasting roles in the survival, motility, and RhoA signaling of astrocytoma cells." in: Experimental cell research, Vol. 316, Issue 7, pp. 1137-47, 2010 (PubMed).
Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).
|Hosts||Rabbit (14), Mouse (4)|
|Reactivities||Human (17), Mouse (Murine) (8), Rat (Rattus) (8), Chicken (3), Cow (Bovine) (2), Dog (Canine) (2), Rabbit (2), Cat (Feline) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (17), ELISA (9), Immunofluorescence (IF) (4), Immunohistochemistry (IHC) (4), Immunoprecipitation (IP) (4), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)|
|Epitopes||N-Term (2), Transcript Variant 2, C-Term (1)|