WAP Four-Disulfide Core Domain 2 (WFDC2) antibody

Antigen: WAP Four-Disulfide Core Domain 2 (WFDC2)

Synonyms: HE4, WAP5, EDDM4, MGC57529, dJ461P17.6, 1600023A02Rik, re4, WFDC2, CE4, WFDC3, MGC127126, BE-20, si:ch211-153a8.3

Clonality: Monoclonal (3F9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN396057

Additional Information

Alternative name: WFDC2

Gene ID: 10406

Immunogen: WFDC2 (AAH46106, 31 a.a. ~ 125 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K

Isotype: IgG2b kappa

Clone: 3F9

Description: Other names: HE4, MGC57529, WAP5, dJ461P17.6WAP four-disulfide core domain 2

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: WFDC2 (AAH46106, 31 a.a. ~ 125 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Molecular Weight: 12993 DA

Comments:

Background: This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Application Details

Application Notes: ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: Clear, colorless solution in phosphate buffered saline, pH 7.2 .

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Detection limit for recombinant GST tagged WFDC2 is approximately 0.03ng/ml as a capture antibody.

Publications

Product: Li, Dowdy, Tipton et al.: "HE4 as a biomarker for ovarian and endometrial cancer management." in: Expert review of molecular diagnostics, Vol. 9, Issue 6, pp. 555-66, 2009 (PubMed).

Montagnana, Lippi, Ruzzenente et al.: "The utility of serum human epididymis protein 4 (HE4) in patients with a pelvic mass." in: Journal of clinical laboratory analysis, Vol. 23, Issue 5, pp. 331-5, 2009 (PubMed).

Xi, LinLin, Ye: "Human epididymis protein 4 is a biomarker for transitional cell carcinoma in the urinary system." in: Journal of clinical laboratory analysis, Vol. 23, Issue 6, pp. 357-61, 2009 (PubMed).

Stein, Hua, Lee et al.: "Voxelwise genome-wide association study (vGWAS)." in: NeuroImage, Vol. 53, Issue 3, pp. 1160-74, 2010 (PubMed).

Hellstrom, Heagerty, Swisher et al.: "Detection of the HE4 protein in urine as a biomarker for ovarian neoplasms." in: Cancer letters, Vol. 296, Issue 1, pp. 43-8, 2010 (PubMed).