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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 43 (DDX43) (AA 2-91) antibody

Details for Product No. ABIN396075, Supplier: Log in to see
Antigen
  • CT13
  • ct13
  • ddx53
  • ENSMUSG00000070291
  • hage
  • HAGE
  • MGC145555
  • OTTMUSG00000019690
  • zgc:174910
Epitope
AA 2-91
4
2
2
1
1
1
1
1
Reactivity
Human
24
9
8
Host
Mouse
18
6
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB)
19
9
9
4
3
2
2
1
Supplier
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Immunogen DDX43 (NP_061135, 2 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G12
Isotype IgG2a kappa
Specificity DDX43 (NP_061135, 2 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DDX43 (DDX43 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 72844 DA
Gene ID 12406
NCBI Accession NP_061135
Research Area Cancer, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is an ATP-dependent RNA helicase in the DEAD-box family and displays tumor-specific expression.

Restrictions For Research Use only
Concentration 0.5mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 43 (DDX43) (AA 2-91) antibody (ABIN396075) Western Blot analysis of DDX43 expression in transfected 293T cell line by DDX43 mono...
Background publications Mathieu, Linley, Reeder, Badoual, Tartour, Rees, McArdle: "HAGE, a cancer/testis antigen expressed at the protein level in a variety of cancers." in: Cancer immunity, Vol. 10, pp. 2, 2010 (PubMed).

Mathieu, Knights, Pawelec, Riley, Wernet, Lemonnier, Straten, Mueller, Rees, McArdle: "HAGE, a cancer/testis antigen with potential for melanoma immunotherapy: identification of several MHC class I/II HAGE-derived immunogenic peptides." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 12, pp. 1885-95, 2007 (PubMed).

Roman-Gomez, Jimenez-Velasco, Agirre, Castillejo, Navarro, San Jose-Eneriz, Garate, Cordeu, Cervantes, Prosper, Heiniger, Torres: "Epigenetic regulation of human cancer/testis antigen gene, HAGE, in chronic myeloid leukemia." in: Haematologica, Vol. 92, Issue 2, pp. 153-62, 2007 (PubMed).

Kimura, Wakamatsu, Suzuki, Ota, Nishikawa, Yamashita, Yamamoto, Sekine, Tsuritani, Wakaguri, Ishii, Sugiyama, Saito, Isono, Irie, Kushida, Yoneyama, Otsuka, Kanda, Yokoi, Kondo, Wagatsuma, Murakawa et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).