DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 43 (DDX43) (AA 2-91) antibody

Details for Product No. ABIN396075
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Antigen
Synonyms ct13, hage, ddx53, MGC145555, zgc:174910, CT13, HAGE, ENSMUSG00000070291, OTTMUSG00000019690
Epitope
AA 2-91
(12), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(22), (14), (14), (12)
Host
Mouse
(20), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(11), (10), (4), (4), (2), (2), (2), (1)
Pubmed 5 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN396075
450.00 $
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Immunogen DDX43 (NP_061135, 2 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3G12
Isotype IgG2a kappa
Specificity DDX43 (NP_061135, 2 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name DDX43
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 72844 DA
Gene ID 12406
NCBI Accession NP_061135
Research Area Cancer, Cell Structure
Application Notes Western blot = 1:500-1000
Comment

Background: The protein encoded by this gene is an ATP-dependent RNA helicase in the DEAD-box family and displays tumor-specific expression.

Restrictions For Research Use only
Concentration 0.5mg/ml
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 43 (DDX43) (AA 2-91) antibody Western Blot analysis of DDX43 expression in transfected 293T cell line by DDX43 monoclonal antibody (M07), clone 3G12. Lane 1: DDX43 transfected lysate(72.9 KDa). Lane 2: Non-transfected lysate.
Background publications Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Roman-Gomez, Jimenez-Velasco, Agirre et al.: "Epigenetic regulation of human cancer/testis antigen gene, HAGE, in chronic myeloid leukemia." in: Haematologica, Vol. 92, Issue 2, pp. 153-62, 2007 (PubMed).

Mathieu, Knights, Pawelec et al.: "HAGE, a cancer/testis antigen with potential for melanoma immunotherapy: identification of several MHC class I/II HAGE-derived immunogenic peptides." in: Cancer immunology, immunotherapy : CII, Vol. 56, Issue 12, pp. 1885-95, 2007 (PubMed).

Mathieu, Linley, Reeder et al.: "HAGE, a cancer/testis antigen expressed at the protein level in a variety of cancers." in: Cancer immunity, Vol. 10, pp. 2, 2010 (PubMed).

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